| 摘要: |
| 已肌苷产生菌枯草杆菌GMI-741(Ade-)为出发菌株,通过多因子诱变选育出具有非精确嘌呤缺陷型、丧失腺嘌呤脱氨酶的AICAR(5-氨基-4-氨甲酰咪唑核苷)产生菌AIC-90,该菌株摇瓶发酵72 h产AICAR可达20.3 g/L以上. |
| 关键词: 枯草杆菌 AICAR 菌种选育 发酵条件 |
| DOI： |
| 分类号:TQ920.1 |
| 基金项目: |
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| Screening of An AICAR-Producing Strain AIC-90 |
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| Abstract: |
| An inosine-producing strain GMI-741 was mutagenized for AICAR-producing strain. A non-exacting purine-requiring mutant AIC90, lacking of adenase, produced a large amount of AICAR(5-amino-4-imidazole-carboxamide riboside). On a reciprocal shaker for 72h, the accumulation of AICAR by AIC90 in the culture broth was more than 20.3g/L. |
| Key words: Bacillus subtilis AICAR strain screening |
