| 摘要: |
| 以人血清为原料,利用纤溶酶原对L型赖氨酸的高亲和性制备了Lysine-Sepharose4B和Lysine-Agarose,以亲和层析法从人血浆中提取和纯化血纤溶酶原(plasminogen,PGn).利用聚丙烯酰胺凝胶电泳对其纯度和分子量进行分析,结果表明纯化得到的为92kDa的单一组分的人血纤溶酶原.这种纯化方法的建立为进一步大量制备血管生成抑制素(angiostatin)奠定了基础. |
| 关键词: 血纤溶酶原 血管生成抑制剂 亲和层析 |
| DOI： |
| 分类号:R931.6 |
| 基金项目: |
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| Isolation and Purification of Plasminogen from Human Plasma |
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| Abstract: |
| Plasminogen (PGn) was extracted and purified from human plasma by Lysine-Sepharose 4B affinity chromatography, which was depended on the high affinity of plasminogen and lysine. Its purity and molecular weight were detected by SDS-PAGE.The result showed that the PGn was a single component with a molecular weight of 92kDa.The establishment of the purification process laid a foundation of further study on angiostatin. |
| Key words: plasminogen angiostatin affinity chromatography |
