| 摘要: |
| 对N-酰化肽的合成条件进行了研究，得出较佳的工艺条件为：温度10-25℃，酶解液与脂肪酰氯的摩尔比为0.7∶1，pH值为9.0。温度对氨基氨的转化率影响不大，在实际生产中可选择室温条件。pH值对反应的影响较大，pH9时比pH8时转化率提高近50%。氨基氮与酰氯的配比为0.7时，能使90%的氨基氮转化为酰化肽。 |
| 关键词: 酶水解物 酰化 肽 |
| DOI： |
| 分类号:Q556 |
| 基金项目:河南省科技攻关项目 |
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| Preparation of N-acyl Peptide from Protease Hydrolysate of Rape Seed Cake(Ⅰ)-Optimization of N-acyl Peptide Preparation |
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| Abstract: |
| A process for production N-acyl peptidc were optimized. At theoptimal ratio of protease hydrolysate and RCOCI (0.7∶1), 90% of the protease hydrolyate was converted to N-acyl peptide at 10-25 ℃, pH 9.0. |
| Key words: Protease hydrolysate,Acylation,Peptide |
