| 摘要: |
| T7噬菌体启动子能被T7RNA聚合酶和真核生物RNA聚合酶Ⅱ系统启动转录，为研究两个系统转录的关键碱基，将合成的T7噬菌体启动子-11变异体与报道基因CAT基因连在一起。体内CAT和体外狭缝RNA杂交实验显示：-11碱基是T7RNA聚合酶和真核生物RNA聚合酶Ⅱ系统启动T7启动子的关键碱基之一。 |
| 关键词: T7噬菌体启动子 T7 RNA聚合酶 真核生物RNA聚合酶Ⅱ 转录 |
| DOI： |
| 分类号:Q933 |
| 基金项目:国家自然科学基金 |
|
| The Effects of Mutant T7 Promoter on Transcriptions |
|
|
| Abstract: |
| To determine key bases on T7 promoter recognized by T7 RNApolymerase and eukaryotic RNA polymerase, the base located at -11 of the promoter was replaced with other bases and the mutant promoters were inserted upstream of coding sequences of CAT gene. CAT test in vivo and Slot-Blot test in vitro showed that the promoter could not be transcripted by both T7 RNA polymerase and eukaryotic RNA polymerase Ⅱ.The result suggests that the -11 base might be the key base for T7 RNA polymerase and eukaryotic RNA polymerase Ⅱsystems. |
| Key words: T7 promoter,T7 RNA polymerase,Eukaryotic Rna polymerase Ⅱ,Transcription. |
