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产氨短杆菌GMA-1112利用味精母液发酵生产肌苷
施庆珊,李良秋,林小平,邱玉棠
广东省微生物研究所 广州 510070
摘要:
通过亚硝基胍或60Coγ辐照等选育一株产氨短杆菌GMA-1112(Ade-+Gua-+VB1-+8-AGr+SGr+6-MPr),能以味精母液代替传统肌苷发酵添加酵母粉作为有机营养物,进行肌苷发酵,平均产肌苷率25.40g/L。具有降低发酵成本、提高产肌苷率等优点。
关键词:  产氨短杆菌  肌苷  味精母液
DOI:
分类号:TQ460.38
基金项目:广东省广州市自然科学基金
Inosine Production from Glutamic Acid Mother Liquid by Brevibacterium ammoniagenes
Abstract:
An inosine-producing mutant of BrevibacteriumammoniagenesGMA-1112(Ade-+Gua-+VB1-+8-AGr+SGr+6-MPr) was bred with the treatment of N-methyl-N-nitro-N-nitrosoguanidine (NTG) and 60Cor radiation etc. The inosine fermentation utilizing glutamic acid mother liquid as an organic nutrient instead of yeast powder was optimized. It was found that the strain could accumulate 25.40g/L of inosine in average.
Key words: