| 摘要: |
| 研究发现10种酵母菌株均可将苯甲醛转化为PAC，且苯甲醛对所有酵母的最小抑制作用均在0.2%～0.4%之间。采用温度、uV、DES、LiCI以及LiCI和uV联合诱变结果，获得3个转化活力较高的变异株，其中变异株S.CarlebergensisCPU9507-4-3″连续接种培养五代并进行转化和测试，结果转化活力均较高，表明变异株遗传稳定性良好。 |
| 关键词: 酵母 诱变 苯甲醛 PAC |
| DOI： |
| 分类号:Q933 |
| 基金项目:国家科技攻关项目，江苏省应用基础研究计划项目 |
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| Selection of Phenysacetycarbinol-producing yeast Strain |
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| Abstract: |
| It was found that all of ten yeast strains studied in this papercould transform benzaldhyde into phenylacetycarbinol (PAC). The inhibitive concentration of benzaldhyde on all these yeast strains was between 0.2%-0.4%. With UV,DES,LiCI and UV combining with LiCI as mutagens, three mutants of the yeast with high transformation efficiency were obtained, of which the S. Carlebergensis CPU 9507-4-3” was inoculated and cultivated sequentially for 5 generation for transformation tests. The results indicated that the strains was genetically stable and could efficiently transform benzaldhyde into PAC with high transformation efficiency. |
| Key words: Yeast, Mutation, Benzaldhy, PAC |
