| 摘要: |
| β-石竹烯是一种双环倍半萜类化合物。为研究β-石竹烯在体外对脂多糖诱导炎症的作用效果及作用机制，利用β-石竹烯处理被脂多糖诱导的RAW264.7细胞炎症模型，用MTT法检测β-石竹烯对细胞活力的影响，用ELISA试剂盒检测IL-6、TNF-α炎症因子的含量变化，用qRT-PCR技术检测IL-6、TNF-α、IL-1β、MCP-1因子的mRNA含量变化，用Western blot检测NF-κB信号通路和JNK信号通路关键蛋白表达变化。结果显示，β-石竹烯能够降低细胞中IL-6因子的含量和IL-6、TNF-α、IL-1β、MCP-1因子的表达，抑制炎症信号通路NF-κB和JNK。因此，β-石竹烯可以通过抑制NF-κB和JNK信号通路，降低炎症因子的释放起到抗炎的效果。该研究为β-石竹烯活性的开发提供数据支持，并进一步为β-石竹烯应用于抗炎治疗方面提供了理论依据。 |
| 关键词: β-石竹烯 炎症 小鼠腹腔巨噬细胞（RAW264.7） 炎症因子 信号通路 |
| DOI：10.14188/j.ajsh.20240509002 |
| 分类号:R364.5 |
| 基金项目:厦门市科技局医疗卫生指导项目（3502Z20214ZD1328）；大学生创新创业训练项目（S202212631020）；福建省教育厅中青年项目（JAT210478）；厦门市海洋与渔业局青年科技创新项目（23YYZP004QCB28） |
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| Study on the inhibition of β-caryophyllene in lipopolysaccharide （LPS） induced inflammatory on macrophage |
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YANG Bingye1,2, ZHANG Yanlin1,2, XU Yihong1,2, DAI Yuanhua1,2
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1.School of Pharmacy and Pharmaceutical Sciences， Xiamen Medical College， Xiamen 361023， Fujian， China;2.Xiamen Key Laboratory of Marine Medicinal Natural Products Resources， Xiamen 361023， Fujian， China
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| Abstract: |
| β-caryophyllene is a dicyclic sesquiterpenoid compound. To study the anti-inflammatory effects and mechanism of β-caryophyllene in lipopolysaccharide （LPS）-induced inflammatory， β-caryophyllene was used to treat RAW264.7 cell inflammatory model induced by LPS. The effects of β -caryophyllene on cell viability were detected by MTT assay， and the contents of IL-6 and TNF-α inflammatory factors were detected by ELISA kit. The mRNA levels of IL-6， TNF-α， IL-1β and MCP-1 were detected by qRT-PCR， and the proteins of NF-κB and JNK signaling pathways were detected by Western blot. The results show that β-caryophyllene can decrease the contents of IL-6 and the mRNA levels of IL-6， TNF-α， IL-1β and MCP-1， and decrease the protein contents of P65 and PJNK in RAW264.7 cells. Overall， these results suggest that β-caryophyllene has the anti-inflammatory effects by decreasing the secretion of inflammatory cytokines Il-6， TNF-α， IL-1β and MCP-1 and inhibiting NF-κB and JNK signaling pathways. It provides the the?oretical basis for developing the functional activity of β-caryophyllene， and furtherly provides a reference for the use of β-caryophyllene in the treatment of inflammation-related diseases. |
| Key words: β-caryophyllene inflammation mouse peritoneal macrophages （RAW264.7） inflammatory cytokine signaling pathway |
