| 摘要: |
| 植物根际微生物在消落区根际生态系统中发挥着重要作用，本研究以此为指针来分析引江济巢工程（菜子湖线）运行前对生态系统的影响。采用16S rRNA基因测序技术对引江济巢工程沿线4个不同区域的植物根际微生物及其环境因子开展了系统调查，在整个调查区域共检出隶属75门（古细菌3门、细菌72门）的根际微生物，其中优势门25门，最丰富的为变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）和酸杆菌门（Acidobacteria）。4个区域之间根际微生物群落组成虽有明显差异，但仍主要是以变形菌门、拟杆菌门、酸杆菌门或放线菌门（Actinobacteria）等为主。单因素方差分析结果表明，4个区域根际微生物Chao1指数、香农指数和Goods coverage指数均存在显著差异，其中巢湖和白石天河区域Chao1指数和香农指数均最低。冗余分析表明，基质pH和有机质质量分数是影响引江济巢沿线根际微生物群落结构的主要环境因子。本研究首次揭示了引江济巢工程运行前植物根际微生物的群落结构及其空间变化，相关结果可为后续评估引江济巢工程运行对沿线植物根际微生物群落的影响提供本底数据。 |
| 关键词: 根际微生物 引江济巢 多样性 群落结构 |
| DOI：10.14188/j.ajsh.20240126001 |
| 分类号:Q939.96 |
| 基金项目:安徽省重点研究与开发计划项目（2022107020002） |
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| Characteristics and main influencing factors of plant rhizospheric microbial communities in riparian zone along the project of water diversion from Yangtze River to the Chaohu Lake |
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GUO Wenxuan1, CHANG Jielun1, LI Bowen1, YANG Lehui1, SHEN Junjie1, WANG Xinyue1, WANG Huili1,2, ZHANG Xiaoke1,2,3
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1.College of Life Sciences， Anqing Normal University， Anqing 246133， Anhui， China;2.Engineering Technology Research Center for Aquatic Organism Conservation and Water Ecosystem Restoration in University of Anhui Province， Anqing 246133， Anhui， China;3.International Joint Research Center of Simulation and Control for Population Ecology of Yangtze River in Anhui， Anqing 246133， Anhui， China
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| Abstract: |
| Rhizospheric microbes of plants play an important role in the rhizosphere ecosystem in the riparian zone. In order to understand the characteristics and main influencing factors of plant rhizospheric microbial communities before the operation of the project of water diversion from Yangtze River to the Chaohu Lake （Caizihu line）， a systematic survey of plant rhizospheric microbial communities and environmental factors in four different regions along this project was conducted. The results showed that a total of 75 phyla （3 phyla of archaea and 72 phyla of bacteria） of rhizospheric microbes were detected in the whole survey area， of which 25 phyla were dominant， and the Proteobacteria， Bacteroidetes， and Acidobacteria were the most abundant. Although there were significant differences in the composition of rhizospheric microbial communities among the four regions， they were still mainly composed of Proteobacteria， Bacteroidetes， Acidobacteria， or Actinobacteria. The results of one-way ANOVA showed that there were significant differences in Chao1， Shannon， and Goods coverage index of rhizospheric microbes in the four regions， among which Chao1 and Shannon index were the lowest in the Chaohu Lake and Baishitianhe River regions. Redundancy analysis showed that substrate pH and organic matter mass fraction were the main environmental factors affecting the rhizospheric microbial community structure along the project. This study revealed the community structure and spatial variation of plant rhizospheric microbial community structure before the operation of the project of water diversion from Yangtze River to the Chaohu Lake for the first time. The relevant results can provide background data for the subsequent evaluation of the impact of the operation of this project on the rhizospheric microbial communities. |
| Key words: rhizospheric microbe the project of water diversion from Yangtze River to the Chaohu Lake diversity community structure |
