| 摘要: |
| 为了筛选对人体常见病原菌具有显著抑菌活性的放线菌资源，本研究采用六种培养基（GA、CMKA、GW、MM、SCK、HV）对新疆乌鲁木齐南山地区土壤样本中放线菌资源进行分离、并选取8株临床标准致病菌株和1株分离自临床耐药金黄色葡萄球菌RS作为指示菌，通过平板对峙法进行抑菌活性放线菌的筛选，最后对具有显著抑菌活性的放线菌进行菌落形态观察和基于16S rRNA序列的系统进化树分析。在分离的109株放线菌中筛选得到3株（C2-4、D4-2和E1-2）均对多重耐药性金黄色葡萄球菌RS（对苯唑西林、四环素、红霉素及青霉素G均有耐药性）、表皮葡萄球菌（Staphylococcus epidermidis）和金黄色葡萄球菌（Staphylococcus aureus）具有显著抑菌活性的放线菌菌株。系统进化树分析表明，放线菌C2-4和D4-2与拟无枝酸菌属（Amycolatopsis）放线菌亲缘关系最近，放线菌E1-2与链霉属（Streptomyces）放线菌进化关系较近。抑菌活性放线菌C2-4、D4-2和E1-2的鉴定，将为其活性次级代谢产物的分离奠定了基础。 |
| 关键词: 放线菌 病原菌 抑菌活性 系统进化树 |
| DOI：10.14188/j.ajsh.2022.04.007 |
| 分类号:Q939.92 |
| 基金项目:2022年度新疆维吾尔自治区自然科学基金（2022D01C182）；陕西省教育厅2021年度一般专项科研计划项目（21JK0639） |
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| Isolation， screening and identification of three strains of actinomycetes resistant to multi drug resistance Staphylococcus aureus |
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QIN Peigang1, LI GongKui1, DAI Xiaojuan2, GUAN Peng2
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1.College of Basic Medicine， Xinjiang Medical University， Urumchi 830011， Xinjiang， China;2.Medical College， Weinan Vocational and Technical College， Weinan 714026， Shaanxi， China
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| Abstract: |
| To screen actinomycetes with antibacterial activity against common pathogenic bacteria in human body， six media were used for isolating of actinomycetes resources from soil samples of the Nanshan district of Xinjiang. Eight clinical standard pathogenic strains and one multidrug resistant Staphylococcus aureus which was isolated from clinical practice were used as indicators to screen antibacterial activity by plate confrontation assay. Finally， the colony morphology of strains with significant antibacterial activity was observed， and phylogenetic tree analysis based on 16S rRNA sequence was carried out. Three antibacterial strains （C2-4， D4-2 and E1-2） were obtained from 109 isolates， which showed strong inhibition on the multidrug resistant Staphylococcus aureus （resistance to tetracycline and vancomycin）， staphylococcus epidermidisand Staphylococcus aureus. Phylogenetic tree analysis confirms that C2-4 and D4-2 strains have the closest relatives with Amycolatopsis strain， and E1-2 strain is closely related to Streptomyces strain. Identification of the C2-4， D4-2 and E1-2 will provide foundation for isolation of their active secondary metabolites. |
| Key words: actinomycete pathogen antibacterial activity phylogenetic tree |
