| 摘要: |
| 通过抑菌及细胞壁降解酶活性试验，研究向日葵花盘（sunflower disc，SFD）水提物对引起马铃薯干腐病主要病原菌——硫色镰刀菌（Fusarium sulphureum）生长及其侵染不同马铃薯品种时分泌的多聚半乳糖醛酸酶（polygalacturonase，PG）、果胶甲基半乳糖醛酸酶（polymethyl-galacturonase，PMG）、纤维素酶（carboxymethyl cellulose，Cx）、β-葡萄糖苷酶活性变化。结果表明，向日葵花盘水提物对硫色镰刀菌生长及侵染陇薯7号、陇薯10号、新大坪3个马铃薯品种分泌的4种细胞壁降解酶活性均具有抑制作用，最佳抑制浓度为320 mg/mL，在0~640 mg/mL浓度内，随浓度增大，对硫色镰刀菌生长的抑制作用增强；经最佳抑制浓度处理能使陇薯7号的PG和β-葡萄糖苷酶活性降低到未侵染水平，但β-葡萄糖苷酶活性的降低未达到极显著效果，该处理不能使陇薯7号的PMG和Cx及陇薯10号、新大坪的4种细胞壁降解酶活性降低到未侵染水平。 |
| 关键词: 向日葵花盘 硫色镰刀菌 马铃薯干腐病 细胞壁降解酶 |
| DOI：10.14188/j.ajsh.2022.01.012 |
| 分类号:TS209 |
| 基金项目: |
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| Inhibitory characteristics of water extract of sunflower disc on Fusarium sulphureum and its cell wall degrading enzyme activity |
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WANG Cong1, LIU Qing2, ZHAO Ping2, BAI Jie2, YANG Qianqian2, ZHANG Xiuli2, WANG Wenqian2, XIAOKAITI Tuergong2, LIU Bing2, Zou Zhicheng2
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1.Engineering Technology Group Co.，Ltd， Henan College of Transportation ， Zhengzhou 450046， Henan， China;2.School of Life Science and Technology， Lanzhou University of Technology， Lanzhou 730050， Gansu， China
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| Abstract: |
| By methods of bacteriostatic test and cell-wall degrading enzyme activity test， this study investigated the biological control effect of water extract from sunflower disc on Fusarium sulphureum and the activities of 4 cell-wall degrading enzymes. Polygalacturonase（PG）， polymethyl-galacturonase（PMG）， carboxymethyl cellulose（Cx） and β-glucosidase were 4 cell-wall degrading enzymes secreted by Fusarium sulphureum invading the different potato varieties. Results show that the extract is of inhibition effects on Fusarium sulphureum and activities of 4 cell-wall degrading enzymes secreted by Fusarium sulphureum invading potato tubers from 3 potato varieties of Longshu No. 7， Longshu No. 10 and Xindaping， and the best inhibition occurrs at the concentration of 320 mg/mL. With the increasing of concentration in the range of 0 to 640 mg/mL， the inhibition effect on Fusarium sulphureum increased. Treated by the extract of the best concentration， the activities of polygalacturonase（PG） and β-glucosidase of Longshu No. 7 can be reduced to the uninfected level， but the reduction of β-glucosidase activity did not achieve a very significant effect. The activities of polymethyl-galacturonase（PMG） and carboxymethyl cellulose（Cx） of Longshu No. 7 and the activities of 4 cellwall degrading enzymes of Longshu No. 10 and Xindaping could not be reduced to uninfected levels. |
| Key words: sunflower disc Fusarium sulphureum potato dry rot cell wall degrading enzyme |
