| 摘要: |
| 自然条件下，微生物以一种复杂的群落形式生活，细胞周围充斥着由相邻细胞产生的各类代谢物，使各细胞间存在多样的互作形式，影响彼此的生长。不同种类的菌株共培养时，营养缺陷型菌株可以利用其他菌株产生的代谢产物进行生长；共培养还可以改变微环境、刺激菌株沉默基因的表达及改变菌株的生存状态。近年来，基于模拟菌株间的互作关系而发展起来的共培养技术逐步应用于未培养微生物的分离工作中，并被认为能有效提高未培养微生物的分离效率。结合已发表的相关文献资料，综合分析潜在共培养的类群多样性以及共培养分离技术的先进性与应用现状等，以期为微生物分离技术的发展及微生物资源的发掘提供参考。 |
| 关键词: 未培养微生物 互作 共培养技术 分离 培养 |
| DOI：10.14188/j.ajsh.2021.06.009 |
| 分类号:Q93 |
| 基金项目:连云港职业技术学院校级课题（XYB202004） |
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| Advances of co-cultivation and isolation technology based on microbial interaction |
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WANG Chong1, WAN Hongshan1, CHANG Yaqi2, SUN Xujun1, MA Zeliang1
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1.Lianyungang Technical College，Lianyungang 222006， Jiangsu， China;2.Zhengda Tianqing Pharmaceutical Group Co.， Ltd.， Lianyungang 222062， Jiangsu， China
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| Abstract: |
| It is widely recognized that bacteria in nature exist in complex communities. Each cell may be filled with a variety of metabolites produced by neighboring cells， so that there are various forms of interaction between cells. Studies have shown that auxotrophic strains can use metabolites produced by other strains for growth when strains are co-cultured. In addition， co-cultivation can also change the microenvironment of the media， stimulate the expression of the silent gene of the strain and change the dormant state of the strain. In recent years， co-cultivation technology that simulates the interaction between microorganisms has been gradually applied to the isolation of microorganisms， and this method is considered to be effective in isolating uncultivated microorganisms. According to the published literature， we review the research progress of co-cultivation and isolation technology in recent years through comprehensive analysis of the main potential co-cultivable taxa diversity and the application status of co-cultivation technology used for the isolation of uncultivated microorganisms. We hope that this article can provide new prospects for microbial isolation technology and the discovery of microbial resources. |
| Key words: uncultured microorganism interaction co-cultivation technology isolation cultivation |
