| 摘要: |
| 为了研究草酸二甲酯吸入染毒对小鼠的急性毒性，观察吸入染毒后小鼠活动状况、记录心电图、计算LD50，实验结束后取血、检测血液学、血液cTnI和生化指标；取脏器，计算脑、心、肝、脾、肺、肾、卵巢、睾丸、附睾脏器系数，观察其病理组织学改变。结果显示，小鼠吸入草酸二甲酯后出现抽搐、烦躁不安、心电图异常等中毒反应，LD50为2.065 4×10-4 g/cm3，脑、心、肝、脾、肺、肾、睾丸、卵巢脏器系数明显升高，血液学、血生化指标和cTnI水平出现了异常变化，与对照组比较具有显著性差异（P<0.05或P<0.01）；心、肺、肝、小肠和肾组织出现了严重受损。由此可见，草酸二甲酯吸入染毒具有较强的毒性，可引起小鼠心电图、血液中cTnI水平、血液学和血生化指标异常改变，可导致心、肺、肝、小肠和肾脏出现病变。本实验为乙二醇合成过程中产生的碳酸二甲酯职业危害防治提供实验依据。 |
| 关键词: 草酸二甲酯 小鼠 急性毒性 |
| DOI：10.14188/j.ajsh.2021.05.014 |
| 分类号:R965 |
| 基金项目:中国石油化工股份有限公司湖北化肥分公司合作项目（SDHZ2019079）；三峡大学硕士学位论文培优基金（2020SSPY144，2019SSPY159） |
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| Study on subacute inhalation toxicity of dimethyl oxalate in mice |
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KANG Fan1,2, QIAO Qianli1, CAI Gongyu1, YE Rongjun1, FU Zhihua1, WANG Xuewen1, LEI Qiankun2, XU Dengqing2, ZOU Kun2, HE Haibo2
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1.Hubei Chemical Fertilizer Branch of Sinopec Group， Yichang 443200， Hubei， China;2.College of Biological and Pharmaceutical Sciences， China Three Gorges University， Yichang 443002， Hubei， China
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| Abstract: |
| To study the acute toxicity of dimethyl oxalate inhalation in mice， the activity of mice after inhalation was observed， ECG was recorded and LD50 was calculated. After the experiment， the blood samples were taken to detect hematology， cTnI and biochemical indexes. The coefficients of brain， heart， liver， spleen， lung， kidney， ovary， testis and epididymis were calculated. After inhalation of dimethyl oxalate， mice developed convulsions， restlessness， abnormal ECG and other toxic reactions， LD50 was 2.0654×10-4 g/cm3. The organ coefficients of brain， heart， liver， spleen， lung， kidney， testis and ovary were significantly increased， hematology， blood biochemical indexes and cTnI levels showed abnormal changes， and there were significant differences compared with the control （P<0.05 or P<0.01）. The heart， lung， liver， small intestine and kidney were severely damaged. The inhalation of dimethyl oxalate has strong toxicity， which can cause abnormal changes of ECG， cTnI level in blood， hematology and blood biochemical indexes in mice， and lead to pathological changes of heart， lung， liver， small intestine and kidney. This experiment provides experimental basis for the prevention and control of occupational hazards of dimethyl carbonate produced in the process of ethylene glycol synthesis. |
| Key words: dimethyl oxalate mouse acute toxicity |
