| 摘要: |
| 本文以异育银鲫为研究对象，将其在4 ℃水温条件下暂养12 h，研究其脂肪组织在低温环境下的基因表达变化。HE染色切片和透射电镜拍照结果显示，并未观察到由低温处理导致的组织学显著变化，如明显的线粒体结构增多现象。利用Western blot分析手段，检测了常温和低温处理异育银鲫腹部脂肪组织中的线粒体蛋白分子COX IV的表达水平，发现经过低温处理，异育银鲫脂肪组织中的线粒体蛋白COXIV略有增加。荧光定量PCR的结果显示，在低温环境下，异育银鲫腹部脂肪组织中的Cell Death Inducing DFFA Like Effector A（cidea）， Peroxisome proliferator-activated receptor gamma coactivator-1 alpha（pgc1）， Peroxisome Proliferator Activated Receptor Gamma（pparg）， Peroxisome proliferator-activated receptor gamma coactivator 1-beta（ppargca1）， uncoupling protein 1（ucp1）， uncoupling protein 2（ucp2）和uncoupling protein 3（ucp3）等基因的转录表达均出现了显著上调。这是首次证实鱼类腹部脂肪组织中的UCP分子均可以被低温诱导上调表达。 |
| 关键词: 低温处理 脂肪 异育银鲫 基因表达 |
| DOI：10.14188/j.ajsh.2020.06.012 |
| 分类号:Q754 |
| 基金项目:国家重点研发计划（2018YFD0900404） |
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| Effect of cold treatment on the expression of genes related to energy metabolism in abdominal adipose tissue of gible carp |
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LI Wei1,2, HE Jiangyan2, ZHAI Gang2,3, HU Yucai1, YIN Zhan2,3
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1.Dalian Ocean University， Dalian 116000， Liaoning， China;2.Institute of Hydrobiology， Chinese Academy of Sciences， Wuhan 430070， Hubei， China;3.University of Chinese Academy of Sciences， Beijing 100000， China
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| Abstract: |
| In the present study， cold treatment （4 ℃ for 12 hours） has been conducted on gibel carp （Carassius auratus gibelio）， and no significant histological changes in gibel carp visceral adipose tissue （VAT） has been observed. To investigate the expression variation of genes related to energy metabolism， utilizing Western blot analysis， slightly elevated levels of mitochondrial protein COX IV in gibel carp VAT could be observed after cold treatment. In fact， the levels of the transcriptional expressions of cidea， pgc1a/b， pparg， sirt3， ucp1， ucp2 and ucp3 in the gibel carp VAT after cold treatment have been significantly elevated in real-time reverse transcriptase PCR test. These results indicates that the elevated lipid metabolism activities in gibel carp VAT can be induced by cold treatment. |
| Key words: cold treatment fat gibel carp gene expression |
