| 摘要: |
| 恰玛古（Qamgur， Brassica rapa L.）内生菌的研究主要集中在内生真菌，内生放线菌的研究报道较少。通过研究新疆药食两用植物恰玛古内生放线菌多样性，以期发现产新活性物质的放线菌或新种放线菌，为研究微生物药物奠定基础。从恰玛古根、茎和叶三个部位分离培养获得内生放线菌，对其菌落与个体形态进行观察，并利用序列测定方法进行鉴定，以获取其分类地位。从恰玛古三个部位共分离得到17株内生放线菌，其中12株为革兰氏阳性杆菌，3株为革兰氏阳性球菌，2株为革兰氏阳性丝状菌；17株内生放线菌分属于红球菌属（Rhodococcus）、拟诺卡氏菌属（Nocardiopsis）、链霉菌属（Streptomyces）、短杆菌属（Brevibacterium）、小短杆菌属（Brachybacterium）、两面神菌属（Janibacter）和微杆菌属（Microbacterium）。从新疆药食两用植物恰玛古中分离获得17株内生放线菌以稀有放线菌为主。 |
| 关键词: 恰玛古 内生放线菌 分离鉴定 |
| DOI：10.14188/j.ajsh.2020.06.011 |
| 分类号:R379.1 |
| 基金项目:新疆维吾尔自治区自然科学基金（2017D07C212） |
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| Isolation and identification of endophytic actinomycetes from medicinal and edible plant Qamgur in Xinjiang |
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ZHEN Qian1, SUN Yuping2, WEI Jie3, ZHANG Xiaobo3, XIONG Wenjuan1, MA Yan4, NI Ping2, CHEN Feng2
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1.Department of Clinical Laboratory，the Fifth Affiliated Hospital of Xinjiang Medical University， Urumqi 830011， Xinjiang， China;2.College of Basic Medical，Xinjiang Medical University，Urumqi 830011， Xinjiang， China;3.School of Public Health，Xinjiang Medical University，Urumqi 830011， Xinjiang， China;4.Department of Clinical Laboratory，the Second Affiliated Hospital of Xinjiang Medical University， Urumqi 830063， Xinjiang， China
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| Abstract: |
| Endophytic actinomycetes can produce a variety of secondary metabolites and are important sources of new antibiotics. At present， the study of the endophytic actinomycetes from Qamgur （Brassica rapa L.） mainly focuses on endophytic fungi， and there are few reports of endophytic actinomycetes. In order to find new actinomycetes or new active substances， the diversity of endophytic actinomycetes from Qamgur， a dual-purpose plant for medicine and food in Xinjiang， was studied. Endophytic actinomycetes were isolated and cultured from the roots， stems and leaves of Qamgur， subjected to morphological observation， 16S rDNA sequencing and phylogenetic tree analysis. 17 strains were isolated， and 12 were G+ bacilli， 3 were G+ cocci and 2 were G+ filaments. Sequence analysis and phylogenetic tree show that 17 strains belong to Rhodococcus， Nocardiopsis， Streptomyces， Brevibacterium， Brachybacterium， Janibacter and Microbacterium， and most of them are rare actinomycetes. |
| Key words: Brassica rapa L. endophytic actinomycetes isolation and identification |
