| 摘要: |
| 芽胞杆菌因其具有广谱的抑菌活性，在农业生产上常用于防控植物病害。本研究比较了五种芽胞杆菌发酵上清液对禾谷镰刀菌PH-1的抑制作用，结果发现枯草芽胞杆菌SCK6基本没有抑制效果，地衣芽胞杆菌BSK3和多粘芽胞杆菌BSK4有较弱的抑制作用，解淀粉芽胞杆菌TZ01和贝莱斯芽胞杆菌SH06抑制效果明显，其中贝莱斯芽胞杆菌SH06的抑制作用最为显著。进一步从不同培养基、培养时间及渗透剂对SH06菌株发酵条件进行了优化，结果表明在TB培养基中培养24 h并加入0.5% TritonX-100的渗透剂时，发酵上清对禾谷镰刀菌PH-1的抑制效果最佳。本文为贝莱斯芽胞杆菌作为一种新型生防菌剂奠定了良好的应用基础，不仅丰富了生物防治菌种的菌库，也为小麦赤霉病的防治提供了一种新的选择，对未来菌种联合防治提供了新的方向。 |
| 关键词: 贝莱斯芽胞杆菌 禾谷镰刀菌 抑菌活性 发酵条件 生物防治 |
| DOI：10.14188/j.ajsh.2020.04.012 |
| 分类号:Q939.92 |
| 基金项目:苏垦农发农业科技项目（苏垦农发种[2019]125号）；武汉市黄鹤英才（2016） |
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| Screening and related research of Bacillus for the biocontrol of wheat scab |
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JIANG Tianzhi1, HAN Ruqing2, ZHOU Ming2, ZHOU Chen1, SUN Xuehua2, ZHANG Guimin1
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1.College of Life Sciences, Hubei University, Wuhan 430062, Hubei, China;2.Huai’an Dahua Bio-technology Co., Ltd., Huai’an 223216, Jiangsu, China
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| Abstract: |
| Bacillus is widely used in agricultural production to prevent and control plant diseases because of its broad-spectrum antibacterial activity. In this study, the inhibitory effects of fermentation supernatants from five common Bacillus on Fusarium graminearum PH-1 were investigated, respectively. The results showed that B. subtilis SCK6 had no inhibitory effect, and B. licheniformis BSK3 and B.polymyxa BSK4 had weak inhibition. The inhibitory effects of B. amyloliquefaciens TZ01 and Bacillus velezensis SH06 were obvious, and the inhibition of B.velezensis SH06 was the best.Furthermore, the fermentation parameters including medium composition, culture time and different permeability additives on B.velezensis SH06 were optimized. The inhibitory activity against PH-1 reached the highest when cultured SH06 in TB medium for 24 h and supplemented by 0.5% TritonX-100. This study lays a foundation that B. velezensis can be used as a biocontrol agent against plant diseases and can play a significant role in the agriculture economy. |
| Key words: Bacillus velezensis Fusarium graminearum antimicrobial activity fermentation condition biological control |
