| 摘要: |
| Squamosa启动子结合类蛋白(SPL)基因家族编码一类植物特有的转录因子，其功能涉及作物遗传改良的许多方面，如产量、株型、抗逆性等，具有重要的实际应用价值。虽然SPL基因在很多作物中有广泛研究，但是在高粱中仍有待进一步探索。本研究通过生物信息学方法，利用同源序列法在高粱基因组水平对SPL基因家族进行分离和分析，共获得19个高粱SPL基因，并命名为SbSPL。高粱SbSPL家族基因不均匀地分布于高粱9条染色体上。通过系统进化树、保守结构域和基因结构等分析，将SbSPL基因家族成员分为5组，不同组的SbSPL基因在功能结构上具有保守性。此外，分析了SbSPL基因家族成员的启动子，发现SbSPL基因家族具有响应非生物胁迫相关信号转导的顺式作用元件。利用qRT-PCR技术，发现部分高粱SbSPL基因的表达受干旱胁迫诱导。这些结果揭示了SbSPL基因可能在高粱响应环境非生物胁迫过程中起到重要作用。 |
| 关键词: 高粱 Squamosa启动子结合类蛋白基因 转录因子 干旱胁迫 |
| DOI：10.14188/j.ajsh.2020.04.011 |
| 分类号:Q755 |
| 基金项目:三峡大学高层次人才科研启动基金（2016GCRC02） |
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LU Yelei1, DENG Wei1, WANG Zhen1, DU Xiaoyun1, Yang Lü1, HAN Shaopeng1, ZHOU Chao1, ZENG Gongjian1, SHEN Xiangling1
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Key Laboratory of Three Gorges Regional Plant Genetics & Germplasm Enhancement, Biotechnology Research Center, Three Gorges University, Yichang 443002, Hubei, China
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| Abstract: |
| Squamosa promoter binding protein-like (SPL) gene family encodes a class of plant-specific transcription factors, and its functions involve many aspects of crop genetic improvement, such as yield, plant type, stress resistance, and has important practical application values. Although the SPL gene has been extensively studied in many crops, it remains to be further explored in sorghum. This study used bioinformatics method to isolate and analyze the SPL gene family at the sorghum genome level using the homologous sequence method. A total of 19 SPL gene family were obtained and named SbSPL. Sorghum SbSPL family genes were unevenly distributed on 9 sorghum chromosomes. At the same time, SbSPL gene family members can be divided into 5 groups through phylogenetic tree, and conserved domain, gene structure, and the conservation of functional structure of SbSPL genes in different groups were analyzed. In addition, by promoter analysis of the SbSPL gene family, it was found that the SbSPL gene family has cis-acting elements in response to abiotic stress-related signal transduction. Using qRT-PCR technology, it was found that the expression of some SbSPL genes was induced by drought stress. These results reveal that the SbSPL gene may play an important role in the response of sorghum to environmental abiotic stress. |
| Key words: Sorghum bicolor SPL transcriptional factor drought stress |
