| 摘要: |
| 生物反应器（bioreactor）是一种以表达目标产物或获得繁殖体为目的的设备系统，包括微生物、动物、植物生物反应器以及相关设备。植物生物反应器（phytobioreactor）是借鉴植物组织培养和微生物发酵原理制作的设备系统。其中，应用较广泛的是间歇浸没式植物生物反应器。与传统植物组织培养相比，该方法具备可换气、无需转接和大容量培养等特点。国内制作的BIOF系列新型植物生物反应器还可以利用串/并联方法，实现更高通量培养能力，其应用于植物种苗繁育、代谢产物的表达、耐盐等变异的定向筛选、植物生长发育的动态分析等方面均具备显著优势。现代植物生物技术在基础研究和产业方面的应用对植物生物反应器提出了新要求，新型生物反应器应用方法的持续改进和设备系统的不断完善，使其成为植物学领域的高效研究平台，并将促进植物育种和植物源化合物的发掘等方面研究效率的提高。 |
| 关键词: 植物组织培养 生物反应器 高通量 药用资源 |
| DOI：10.14188/j.ajsh.2020.01.016 |
| 分类号:Q94 |
| 基金项目: |
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| High-throughput phytobioreactors and their application in the exploration of genetic resources |
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CHEN Jishuang1,ZHANG Benhou1
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Institute of Bioresource Engineering, Nanjing Tech University, Nanjing 211800, Jiangsu, China
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| Abstract: |
| Bioreactor is a kind of system or device designed for the purpose of efficiently expressing target products and/or propagules for microbes, animals and plants. A phytobioreactor is an equipment system based on the principles of plant tissue culture and microbial fermentation. Amongst the various types of plant bioreactors, the temporary immersion bioreactor system (TIBS) is the most widely used. Compared with traditional plant tissue culture, the method has the characteristics of air exchange, no transfer and mass culture. A novel BIOF TIBS system developed in China, that realizes high throughput capacities by using series or parallel connections of bioreactor tanks, is described herein. Its advantages are demonstrated in applications for seedling propagation, expressing of motabolism products, selection of salt-tolerant plant varieties, and dynamic analysis of plant growth and development. More attention has been raised to phytobioreactors as a new platform for plant tissue culturing by the requirements of modern biotechnology. The continual improvement of methods and equipments for new phytobioreactors will provide more efficient research support of plant science, not only for plant breeding, but also for the discovery of plant derived compounds, especially for plant-microbe interaction studies. |
| Key words: plant tissue culture bioreactor high-throughput medicinal resource |
