| 摘要: |
| 评估淋巴瘤细胞MC/CAR、HUT78和RAMOS的CD52抗原呈现稳定性，并比较三种细胞用于抗CD52单抗活性检测中的优劣性。采用免疫荧光法检测淋巴瘤细胞MC/CAR、HUT78和RAMOS的CD52抗原呈现率，分析MC/CAR、HUT78和RAMOS传代培养5~20代CD52抗原呈现稳定性。分别以MC/CAR、HUT78和RAMOS作为抗CD52单抗结合活性和补体依赖细胞毒性的靶细胞进行检测，并比较其优劣性。结果显示，MC/CAR、RAMOS和HUT78的CD52抗原呈现率分别为95.5%、63.2%和38.3%。MC/CAR传代培养5~20代CD52抗原呈现率均大于90%。RAMOS传代培养5~13代CD52抗原呈现率介于60%~67%，第14~15代CD52抗原呈现率介于50%~60%，第16~20代细胞CD52抗原呈现率介于40%~50%。HUT78传代培养5~20代CD52抗原呈现率介于26.7%~38.9%。淋巴瘤细胞MC/CAR在抗CD52单抗的结合活性检测中呈现出更好的剂量依赖曲线。淋巴瘤细胞RAMOS在抗CD52单抗的补体依赖细胞毒性检测中呈现出更好的剂量效应曲线。CD52抗原呈现率方面MC/CAR>RAMOS>HUT78。结果表明，MC/CAR更适用于抗CD52单抗的结合活性的检测，RAMOS适用于抗CD52单抗的补体依赖细胞毒性检测。 |
| 关键词: CD52抗原 HUT78 RAMOS MC/CAR 抗原呈现率 |
| DOI：10.14188/j.ajsh.2019.05.009 |
| 分类号:Q291 |
| 基金项目:兰州市科技重大专项 2017-2-1兰州市科技重大专项（2017-2-1） |
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| Appearance stability of CD52 antigen in three kinds of lymphoma cells and its application |
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QIN Haiyan1,CHEN Jijun1,AN Chen1,XIONG Ying1,YE Xing1,NAN Jianjun1,MAO Xiaoyan1
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Fourth Department of Research, Lanzhou Institute of Biological Products Co., Ltd., Lanzhou 730046, Gansu, China
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| Abstract: |
| To evaluate the appearance stability of CD52 antigen in lymphoma cells MC/CAR，HUT78 and RAMOS, and to compare the advantages and disadvantages of the three cells used in the biological activity detection of anti-CD52 monoclonal antibody, the appearance rate of CD52 antigen in three cells were detected by immunofluorescence. The appearance stability of CD52 antigen in MC/CAR, HUT78 and RAMOS were analyzed for 5~20 generations. The binding activity and complement dependent cytotoxicity of anti-CD52 monoclonal antibody were performed using MC/CAR, HUT78 and RAMOS as target cells, respectively, and their advantages and disadvantages were compared. CD52 antigen appearance rate of MC/CAR, HUT78 and RAMOS were 95.5%, 63.2%, and 38.3%, respectively. CD52 antigen appearance rates of MC/CAR were all > 90% at 5~20 generations. CD52 antigen appearance rate of RAMOS was between 60% and 67% at 5~13 generations, between 50% and 60% at 14~15 generations, and between 40% and 50% at 16~20 generations. CD52 antigen appearance rate of HUT78 was between 26.7% and 38.9% at 5~20 generations. MC/CAR showed a better dose-dependent curve in binding activity of anti-CD52 monoclonal antibodies. RAMOS showed a better dose-response curve in complement dependent cytotoxicity activity of anti-CD52 monoclonal antibody. The appearance rate of CD52 antigen in lymphoma cells were MC/CAR>RAMOS>HUT78. MC/CAR was more suitable for the binding activity detection of anti-CD52 monoclonal antibody. RAMOS was more suitable for complement dependent cytotoxicity detection of anti-CD52 monoclonal antibody. |
| Key words: CD52 antigen HUT78 RAMOS MC/CAR antigen appearance rate |
