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| 来自猪肠道芽胞杆菌培养液中氨基酸成分分析 |
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王升平1,4,刘惠知1,2,周映华1,2,罗开武3,李丽立1,4,张彬1,4,高书峰1,2,周小玲1,2,邬理洋1,2
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1. 湖南农业大学动物科学技术学院,湖南长沙410125;湖南省微生物研究院,湖南长沙410009;2. 湖南省微生物研究院,湖南长沙,410009;3. 湖南省桃源县畜牧兽医水产局,湖南常德,415700;4. 湖南农业大学动物科学技术学院,湖南长沙,410125
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| 摘要: |
| 从动物肠道分离得到112株芽胞杆菌,采用氨基酸全自动分析仪对分离得到的芽胞杆菌的上清进行氨基酸分析.结果表明,不同芽胞杆菌菌株上清中各氨基酸水平相差较大.其中,动物的几种限制性必需氨基酸中,赖氨酸的含量分布在0.18～0.32 g·L-1之间,蛋氨酸的含量分布在0.05～0.09 g·L-1之间.精氨酸的含量分布在0.004～0.02 g·L-1之间.所有氨基酸种类中,赖氨酸、亮氨酸、苯丙氨酸、异亮氨酸含量较为丰富,超过了0.1g·L-1,而天冬氨酸、苏氨酸、丝氨酸含量较少,不到0.01g·L-1.这些结果为开发提高饲料氮利用效率的饲用益生菌提供参考. |
| 关键词: 芽胞杆菌 培养上清 氨基酸 |
| DOI： |
| 分类号:S834.83 |
| 基金项目:国家自然科学基金(31302003)，江西省星火计划项目(20142BBF61051) |
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| Component Analysis of Amino Acids in the Culture Supernatant of Bacillus Strains Isolated from Porcine Intestinal Tract |
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WANG Shengping,LIU Huizhi,ZHOU Yinghua,LUO Kaiwu,LI Lili,ZHANG Bin,GAO Shufeng,ZHOU Xiaoling,WU Liyang,SHU Yan,MIAO Dong,ZENG Fajiao,College of Animal Science,Technology,Hunan Agricultural University
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1.College of Animal Science and Technology,Hunan Agricultural University,2.Hunan Institute of Microbiology,Farming and Veterinary and Aquatic Product Bureau,Taoyuan Country
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| Abstract: |
| One hundred and twelve Bacillus strains were isolated from pig intestine in the present study.The amino acid content in the culture supernatant of 112 Bacillus strains was determined by Automatic Amino Acid Analyzer.The results indicated that there existed large differences of the amino acid content in the culture supernatant among different Bacillus strains.The contents of essential amino acid lysine,methionine were 0.18~0.32g·L-1 and 0.05~0.09g·L-1 respectively.The contents of arginine was 0.004~0.02g·L-1.The contents of lysine,leucine,phenylalanine and isoleucine were more than 0.1g·L-1.The contents of aspartic acid,threonine and serine were less than 0.01g·L-1.The present study provides important references for developing feed probiotics and improving nitrogen use efficiency. |
| Key words: Bacillus culture supernatant amino acid |
