| 摘要: |
| 目的:优选出羊胎免疫调节因子提取的最佳工艺.方法:采用正交实验设计L8(27),以淋巴细胞转化实验为考察指标进行实验,对提取过程中的6个指标进行了优化,确定了最佳提取工艺.结果:羊胎免疫调节因子最佳提取工艺为A1B2C2D1E2F2,即2月龄内羊胎粉碎10 min,加1:4双蒸水常温下8000 r·min-1离心后10K超滤膜超滤即得. |
| 关键词: 羊胎免疫调节因子 提取工艺 正交设计 |
| DOI： |
| 分类号:R318 |
| 基金项目: |
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| Study on Optimum Extraction Process for Goat Embryo Immunoregulatory Factor (GEIF) |
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| Abstract: |
| To study the optimum extraction process for goat embryo immunoregulatory factor(GEIF),the lymphocyte transformation was investiqated.There were 6 indexes in the extraction process by orthogonal design,and then the optimum extraction process was confirmed.The optimum extraction process was A1B2C2D1E2F2,namely,the goat embryo's month age was younger than 2,crushed 10min,added double-distilled water1:4(v/v),normal temperature,centrifuged with 8000g,and obtained 10K molecular weight by ultrafilter. |
| Key words: goat embryo immunoregulatory factor(GEIF) extraction process orthogonal design |
