| 本文已被：浏览 1436次 下载 1158次 |
码上扫一扫！ |
|
|
| S-腺苷-L-蛋氨酸(SAM)稳定性盐产品制备的研究 |
|
刘恭源1,崔海容2,郭坚2,蔡诗贵2,何光源1
|
|
1. 华中科技大学,中英HUST-RRes基因工程和基因组学联合实验室,武汉,430074;2. 湖北出入境检验检疫局技术中心,武汉,430022
|
|
| 摘要: |
| 研究了S-腺苷-L-蛋氨酸(SAM)盐产品的制备方法,确定了甲醇沉淀结晶SAM硫酸盐的最优条件,进一步优化了脱色精制SAM双盐产品的方法.破胞液经超滤后,过D152树脂以硫酸溶液洗脱,可得到纯度(色谱纯)在98%以上的SAM硫酸盐产品,以对甲苯磺酸溶液溶解SAM硫酸盐后脱色精制得到SAM硫酸对甲苯磺酸双盐. |
| 关键词: S-腺苷-L-蛋氨酸(SAM) 沉淀结晶 脱色 冷冻干燥 |
| DOI： |
| 分类号:Q81 |
| 基金项目:国家自然科学基金，国家重点基础研究发展计划(973计划) |
|
| Study on Preparation of Stable Salts of S-Adenosyl-L-Methionine |
|
|
| Abstract: |
| Purification and preparation of stable salts of S-adenosyl-L-methionine were investigated and the optimum conditions for precipitating salts of SAM with methanol had been determined as well as the methods for preparing double salts of SAM had been improved.Being filtrated after breaking yeast cells and purified by D152 resins,eluting with H_2SO_4,purity of SAM(HPLC) could achieve 98%.After dissolving salts of SAM with p-toluene sulfuric acid,p-toluenesulfonate salts of SAM was obtained. |
| Key words: S-adenosyl-L-methionine precipitation decolor freeze-drying |
