| 摘要: |
| 建立了一种简便、准确、可靠的葛根素测定方法.采用Symmetry C18(3.9mm×150mm,5μm)柱,流动相采用甲醇-水(体积比为35:25),流速为1.00ml·min-1,检测波长为250nm.进样量在0.0368μg～0.589μg范围内线性关系良好,(r=0.9994).该方法简便快速,准确可靠,可用于葛根素注射液的含量测定.同时,测定结果表明两种产品在含量上有差别,因此,保证和进一步提高葛根素注射液质量,并且规范质量标准势在必行. |
| 关键词: 葛根素 葛根素注射液 含量测定 HPLC |
| DOI： |
| 分类号:TQ460.7 |
| 基金项目: |
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| Determination of Puerarin Coutent in Puerarin injection by HPLC |
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| Abstract: |
| To establish a method for determination of puerarin, puerarin content of the products was determined by HPLC in Symmetry C18( 3.9mm×150mm,5μm) using a mobile phase of MeOH:H2O=35:65 and a UV detector of 250nm. A standard curve was abtained with a liner range of 0.0368μg～0.589μg(r=0.9994). The method was simple and accurate and could be used for determination of purerarin in puerarin injection. |
| Key words: puerarin puerarin injection content determinarion HPLC |
