| 摘要: |
| 采用柱前衍生高效液相色谱法(AccQ·Tag法)同时测定17种氨基酸,氨基酸浓度在10～100μmol·L-1(胱氨酸浓度在5～50μmol·L-1)时,其峰面积和氨基酸浓度的线性相关系数均在0.99以上,17种氨基酸的加标回收率在92.1%～103.7%之间,用此法测定了绿豆蛋白酶解液氨基酸的含量,取得了满意的结果. |
| 关键词: 高效液相色谱法 氨基酸 AccQ·Tag 酶解液 |
| DOI： |
| 分类号:Q503 |
| 基金项目: |
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| RP- HPLC Determination of Amino Acids in Green Bean Protein Hydrolysate by AccQ. Tag |
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| Abstract: |
| s:17 amino acids in green bean were determined by HPLC AccQ.Tag. When concentrations of amino acids were 10～100μmol·L~ -1 (the cysteine was 5～50μmol·L~ -1), the correlation coefficients between the peak area and the concentration of amino acid were above 0.99. The recoveries of 17 amino acids added were 92.1%～103.7%. The method was applied to amino acids in Green bean protein hydrolysate and good results were obtained. |
| Key words: HPLC amino acid AccQ·Tag green bean protein hydrolysate |
