| 摘要: |
| 从苯乙烯、甲基丙烯酸甲酯、二乙烯苯合成了具有磺酸、羧酸功能基团的新型Dpsc离子交换树脂,并对其表征了选择性、分离度、理论等板高度.用新型Dpsc离子交换树脂在制备规模从发酵缬氨酸液中分离制备了L-缬氨酸,应用0.2 mol·L-1NH4Cl和0.5 mol·L-1NH4OH,流速6.0 mL·min-1,温度25℃,梯度洗脱,减少保留体积4/5. |
| 关键词: 氨基酸 离子交换树脂 制备色谱 发酵缬氨酸 梯度洗脱 |
| DOI： |
| 分类号:TQ92 |
| 基金项目:国家科技攻关项目 |
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| Amino Acid - Preparative Chromatography--Separation of L - Valine Fermented Broth on Dpsc Ion- Exchange Resin Column |
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| Abstract: |
| A new Dpsc ion-exchange resin was synthesized with sulforic acid and carboxylic acid from styrene-methyl methylacrylic acid-divinyl benzene(vinyl styrene). The new Dpsc ion-exchange resin was characterized with the selectivity factor, resolution and The height equivalent to a theoretical plate. The L-valine was prepared with Dpsc ion-exchange resin from valine fermented broth on a preparation scale. The retention volume was reduced to four fifth by 0.2moL/L and 0.5moL/L gradient elution on the new Dpsc ion-exchange resin column at a flow rate of 6ml/min at 25℃. |
| Key words: amino acids ion-exchange resin preparative chromatography L-valine-fermented broth gradient elution. |
