| 摘要: |
| 采用mRNA差异显示RT-PCR方法克隆到人呼吸道合胞病毒感染的SPC-A1细胞中相关的50个表达序列标签,其中一个片段g17-1在HRSV感染的细胞中高表达。利用生物信息学的方法对g17-1进行分析鉴定,推测是一个新基因。利用电子克隆,得到一全长2101bp的cDNA,对其ORF、启动子、电子表达谱、染色体定位以及所编码的蛋白进行分析,结果表明,该cDNA含有1个ORF,与人类假定蛋白XP_097121同源性达90%,定位于人17号染色体,在肿瘤细胞、腺癌细胞、扁桃体初级B细胞中表达,其编码的蛋白预测定位于细胞核内,推测其与细胞抗病毒感染有一定的关系。 |
| 关键词: 生物信息学 人呼吸道合胞病毒 电子克隆 |
| DOI： |
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| In Silico Cloning and Analysis of zzz171con Gene in SPC-A1 Cells Responsive to Human RSV Infection |
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| Abstract: |
| According to mRNA differential display RT-PCR, we have obtained 50 ESTs(Expressed Sequence Tags)from SPC-A1 cells in response to Human RSV Infection, one of which was named g17-1 and highly expressed in SPC-A1.The EST g17-1 was identified to be a novel gene fragment by bioinformatics methods. 2101bp contig product was derived from in silico cloning followed by analysis of open reading frame(ORF), promoter sequence, electronic expression pattern and localization on chromosomeed.The result shows that the contig contained 1 ORF and it was homologous with homo hypothetical protein XP_097121, located on NO. 17 chromosome, and expressed in primary B-cells, carcinoid cells and adenocarcinoma cells. The predictive location of the protein was in nucleus. The cDNA was possibly connected with antiviral functions of SPC-A1 cells. |
| Key words: bioinformatics human respiratory syncytial virus(HRSV) in silico cloning |
