| 摘要: |
| 研究了基因工程菌1016所产的氨基酰化酶的酶学特性.该酶的拆分速率符合米氏方程,且在0.5mol/L的高底物浓度下,无底物抑制现象.37℃时的米氏常数和最大反应速率分别为0.048mmol/L和2.178mmol/L·h.最适反应温度为55℃.55℃时,Km为0.037mmol/L,Vmax为2.558mmol/L·h.最适底物为乙酰蛋氨酸;热稳定性好. |
| 关键词: 氨基酰化酶 动力学 拆分 蛋氨酸 |
| DOI： |
| 分类号:Q814TQ464.7 |
| 基金项目: |
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| Characterization of L-aminoacylase in Bioengineered Bacterium 1016 |
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| Abstract: |
| The aminoacylase characteristics of bioengineered bacterium 1016 were investigated. The results showed that the reaction adhered to Michcelis-Menten equation and there was no substrate inhibition when substrate concentration was not higher than 0.5mol.L -. At 37℃, kinetic constants Km and Vm were 0.048mmol.L - and 2.178 mmol.L -h - respectively. At 55℃,Km was 0.037mmol.L - and Vm was 2.558mmol.L -h -. The optimal substrate and temperature for aminoacylase were N-AC-D,L-Met and 55℃ respectively. The aminoacylase activities could keep steady at higher temperatures. |
| Key words: aminoacylase kinetics resolution L-methionine |
