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化学比色法测定发酵液中L-缬氨酸的研究
李晓华,陈宁,张克旭
天津科技大学食品科学与生物工程学院,天津,300222
摘要:
应用"化学比色法"对发酵液中L-缬氨酸进行了定量分析研究,确立了L -缬氨酸定量测定条件和计算方法.以高速氨基酸分析仪测定结果为参比,用化学比色法测定结果的准确度比纸层析-色斑洗脱比色法高,且应用化学比色法测定L-缬氨酸的含量较为方便.
关键词:  缬氨酸  化学比色法
DOI:
分类号:R12
基金项目:
Study on Chemical Colorimetry Determining L-valine in Fermented Broth
Abstract:
L-valine concentration in fermented broth was quantitatively determined with methods of chemical colorimetry. The quantitative determination conditions and computing method were established. Compared with high-speed amino acid analyzer, the determination value of L-valine concentration with chemical colorimetry was more accurate and convenient than with paper chromatography-mottle elution colorimetry.
Key words:  L-valine  chemical colorimetry