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| 高效液相色谱法测定17AA氨基酸注射液中N-乙酰-L-半胱氨酸和N-乙酰-L-酪氨酸 |
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陈永波1,饶斌2,沈艳芬1,程群1,赵清华1,田远政2
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1. 湖北恩施南方马铃薯研究中心,湖北,恩施,445000;2. 湖北省八峰药化股份有限公司,湖北,鹤峰,445800
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| 摘要: |
| 建立了一种用高效液相色谱测定17AA氨基酸注射液中N-乙酰-L-半胱氨酸和N-乙酰-L-酪氨酸含量的方法.样品经稀释后,直接上机测定,其色谱条件为:Waters Symmetry C18色谱柱,流动相为8.5 mmol/L NaAc-5%CH3OH (pH=4.0),柱温36 ℃,流速1.0 ml/min,检测波长195 nm,N-乙酰-L-半胱氨酸在5.4 mg/L到540 mg/L、N-乙酰-L-酪氨酸在4.9 mg/L到490 mg/L之间,标准曲线呈良好的线性关系(r2>0.9999).该方法具有良好的重现性,日内相对标准偏差小于3%,日间相对平均误差小于7 %,样品回收率在90 %～110 %之间.该方法与测定氨基酸注射液的其它高效液相色谱法相结合,能够对17AA氨基酸注射液中所有氨基酸成分进行全面检测. |
| 关键词: 高效液相色谱 N-乙酰-L-半胱氨酸 N-乙酰-L-酪氨酸 17AA氨基酸注射液 |
| DOI： |
| 分类号:Q503 |
| 基金项目: |
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| Analysis of N-Acetyl-L-Cysteine and N-Acetyl-L-Tyrosine in 17 Amino Acid Injection by High Performance Liquid Chromatography |
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| Abstract: |
| The chromatography conditions were: Waters Symmetry C18 column(3.9mm i.d.×150mm, 4μm) as solid phase, 8.5mmol/L sodium acetate-5% methyl alcohol (pH=4.0) as liquid phase, column temperature of 36℃, flow rate of 1.0ml/min and the detect wavelength of 195nm. The calibration curves of N-acetyl-L-cysteine from 5.4mg/L to 540mg/L and N-acetyl-L-tyrosine from 4.9mg/L to 490mg/L were linear(r 2>0.9999). The analytical method was shown to be highly reproducible, and the absolute recoveries were within 90%-110%. |
| Key words: high performance liquid chromatogram N-acetyl-L-cysteine N-acetyl-L-tyrosine 17AA injection |
