| 摘要: |
| 用(NH4)2SO4分级沉淀、离子交换和分子筛等方法,从食用蘑菇中分离纯化出一种凝集素,经SDS-PAGE测定其亚基的相对分子量为15.8kDa,质谱分析法测定其分子量为32kDa,说明该凝集素由两个亚基组成.氨基酸分析发现,该凝集素不含半胱氨酸.热稳定试验和耐酸碱试验显示,该凝集素活性具很高稳定性. |
| 关键词: 蘑菇凝集素 血凝集活性 生化特征 |
| DOI： |
| 分类号:R977.4 |
| 基金项目:国家自然科学基金 |
|
| Biochemical Characteristics and Amino Acid Analysis of A Novel Agglutinin from Edible Mushrooms |
|
|
| Abstract: |
| From edible mushrooms, a agglutinin was purified by precipitation of saturated (NH 4) 2SO 4 followed by DEAE-Fast Flow and Sephacryl S-200 column chromatography. Its molecular weight (MW) was 32kDa by mass spectrum, and subunit's MW 15.8kDa by SDS-PAGE, indicating that the agglutinin consisted of two subunits. The analysis for its amino acid composition suggested that there wasn't cysteine. The temperature-test and pH-test demonstrated the agglutinin was very stable. |
| Key words: agglutinin erythrocyte agglutination activity biochemical characteristics |
