| 摘要: |
| 以黑糯B糙米皮为实验材料,用1.5mol/L盐酸-95%乙醇(V/V:15/85)溶液提取黑米花青苷色素(BRAP),采用紫外可见分光光度法研究了11种金属离子以及(NH4)1+离子对BRAP的作用.结果表明,未加离子条件下色素溶液可见光区λmax535nm,紫外光区λmax280nm,加入Al3+、Fe3+、Fe2+、Cu2+、Mn2+、Zn2+、Sn2+对其吸收光谱有显性影响.其中Al3+、Fe3+使535 nm特征吸收峰发生蓝移,Sn2+使其发生明显红移;Al3+,Fe2+,Mn2+,Zn2+在535 nm附近有增加ABS值作用,Fe3+有减小ABS值作用;延长作用时间,Cu2+对BRAP吸收光谱的影响表现为λmax535nm发生蓝移,ABS值减小. |
| 关键词: 黑米 花青苷 色素 金属离子 光谱 |
| DOI： |
| 分类号:Q68 |
| 基金项目:陕西省教育厅资助项目 |
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| Influence of Metal Ions on the Absorption Spectrum of Black Rice Anthocyanin Pigment |
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| Abstract: |
| The effect of 11 metal ions and (NH 4) 1+ on black rice anthocyanin pigment(BRAP) was investigated by UV-VIS spectrometry. The pigment solution without metal ions showed the λmax535nm in VIS-region and λmax280nm in UV-region. When Al 3+ ,Fe 3+ ,Fe 2+ ,Cu 2+ ,Mn 2+ ,Zn 2+ and Sn 2+ added separately, the solution was obviously influenced. Among them, the Al 3+ and Fe 3+ made the characteristic absorption peak move to blue λmax 535nm and the Sn 2+ made it red shift. When Al 3+ ,Fe 2+ ,Mn 2+ ,Mn 2+ and Zn 2+ were added in the pigment solution respectively, the ABS value near 535nm was increased, but Fe 3+ made it reduce. Extending the time of Cu 2+ reaction, the pigment solution λ max 535nm shift to blue and the ABS value reduced. |
| Key words: black rice anthocyanin metal ions spectrum |
