| 摘要: |
| 利用引种仙客来的叶片、叶柄、球茎等为外植体进行组织培养与再生植株,筛选最适诱导和继代增殖培养基为MS+BA 2(mg/l,下同)+KT0.2+2,4-D0.5.其中叶片的诱导率和繁殖系数较高,叶柄次之;生根较难;球茎因常内带菌而造成培养困难. |
| 关键词: 仙客来 组织培养 植株再生 |
| DOI： |
| 分类号:Q943.1 |
| 基金项目:陕西省教育厅资助项目，陕西省重点学科项目 |
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| In vitro Cultivation and Rapid Propagation of Cyclamen persicum |
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| Abstract: |
| The leaves, petiole and other explants from Cyclamen persicumas were cultured on MS media containing 2 mg/L of KT, and 0.5 mg/L of 2,4 D to induce callus and to regenerate individual plantlet. It was found that the callus from leaves was easy to induce and grew rapidly. The petiole was difficult to form callus and root and the corm were more difficult to form callus, because of the contamination. |
| Key words: Cyclamen persicum, tissue culture, plantlet regeneration |
