| 摘要: |
| 对拉曼被孢霉突变株F5发酵生产γ-亚麻酸的最适碳源、氮源、发酵时间及温度、无机盐离子添加、最适碳源浓度及补加碳源时间等发酵条件进行了研究探讨.最适发酵培养基组成为(g/L):葡萄糖100,酵母浸出粉4,蛋白胨1,K2HPO4 1,CaCl2 1×10-2,MgSO45×10-2,FeSO4 1×10-2,ZnSO4 7.5×10-3,CuSO4 0.5 × 10-5,MnSO4 2×10-3,pH 6.0.培养温度为25℃,140r/min振荡培养10天,培养8天后(即收获前2天)补加5%葡萄糖.发酵结果为:DC24.59g/L,TL 10.84g/L,TL/DC44.09%,GLA/TL10.67%,GLA产量为1156.63 mg/L. GLA产量较初始结果提高156.15%.该菌株已达到工业化生产菌株要求. |
| 关键词: 拉曼被孢霉 γ-亚麻酸 发酵生产 |
| DOI： |
| 分类号:TQ92 |
| 基金项目:军队科研项目 |
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| Study on the Production of γ-Linolenic Acid by the Fermentation of Moritierella ramanniance Mutant strain F5 |
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| Abstract: |
| The fermentation conditions such as carbon source, nitrogen source, fermentation time, temperature, metal ions supply, the original content of carbon source, the time of carbon source supply, and so on for production of lipid and γ linolenic acid with Moritierella ramanniance mutant strain F 5 were optimized. The optimum culture medium was (g/L):glucose 100, the extract of yeast 4, peptone 1, K 2HPO 4 1, CaCl 2 1×10 -2 ,MgSO 4 5×10 -2 , FeSO 4 1×10 -2 , ZnSO 4 7.5×10 -3 , CuSO 4 0.5×10 -3 , MnSO 4 2×10 -3 , pH 6.0. The optimum culture conditions were: 25 ℃, rotary shaking at 140 min, for 10 days, supplied 5% glucose after cultiration of 8 days. The dry cell weight, lipid yield, the content of lipid, GLA yield and the content of GLA were: 24.59 g/L, 10.84 g/L, 44.09%, 1156.63 mg/L and 10.67%, respectively. The GLA yield was 156.15% more than that at the original condition. The study indicates that the strain can be used for the industrial production of GLA. |
| Key words: Moritierella ramanniance γ linolenic acid fermentation |
