| 摘要: |
| 用不同水解率的水解液合成了N-酰化肽，对比了它们的表面活 性，由碱性蛋白酶水解菜籽蛋白，当水解率为30.6%时，所合成的N-酰化肽性能比水解率为 15.7%、20.3%时要好，用Sephadex G-15分析了水解液中肽分子量的分布情况，发现较佳水 解率的水解液中80%的肽分子量小于1500Da。 |
| 关键词: 菜籽蛋白 酶水解物 酰化 肽 |
| DOI： |
| 分类号:Q556.9 |
| 基金项目:河南省科技攻关项目 |
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| Preparation of N-acyl Peptide from Protease Hydrolysate of Rape Cake(Ⅱ)——Effects of the hydralysis Efficiency |
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| Abstract: |
| In this preliminary study, different hydrolysates of rape cake were compared for their usefulness in preparation of N-acyl peptide. I t was found that rape cake protein hydrolysate prepared with alkaline protease w as the best starting material for synthesis of N-acyl peptide when the hydrolysi s efficiency was 30.6% and if the content of peptides with a molecular weight le ss than 1,5 kD in the lysate was 80%. |
| Key words: Rape cake hydrolysate, acylation, peptide |
