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| 肝素钠树脂精制工艺的研究 |
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江燕1,张力跃2,杨高潮3
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1. 云南大学生物技术系,云南,昆明,650091;2. 中国科学院昆明动物,研究所,昆明,650223;3. 湖北省医药保健品进出口公司,武汉,430022
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| 摘要: |
| 利用D254树脂可以将原始粗品肝素钠的效价提高到179 .8 USP U/mg,进一步纯化可得201.5 USP U/mg的精品,其H2O2用量(1.5%)和氧化时间( 24 h)均少于传统工艺,产品光吸收和收率(85.8%)最优. |
| 关键词: 肝素钠 纯化 D254树脂 |
| DOI： |
| 分类号:Q503 |
| 基金项目: |
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| Study on Purification of Sodium Heparin from the Crude Preparation with Resin D254 |
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| Abstract: |
| A procedure for purifying sodium heparin from its crude preparation was developed. Heparin was adsorbed on resin D 254 under the condition of pH4.0 and 1.5 mole/1 NaCl and was eluted with 3.5 mole/1 NaCl. Heparin in the eluate was precipitated with 45% ethanol. Valence of heparin at this step was increased to 179.8 USP μ/mg. After removing acidic protein with precipitator YNB 99 1 and basic protein with the oxidization of 1.5% H 2O 2 for 24 hours, the valence of hepariu was increased to 201.5 USP μ/mg. Comparing with traditional process, the quality of heparin produced with the new method was the best and the yield was the highest (85.8%). |
| Key words: Heparin sodium Purification Resin D 254 |
