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| 粗品肝素钠质量情况和效价范围的判断 |
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江燕1,张力跃2,杨高潮3,周跃光4
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1. 云南大学生物技术系,云南,昆明,650091;2. 中科院昆明动物所,昆明,650223;3. 湖北省医药保健品进出口公司,武汉,430022;4. 云南省环境科学研究所,昆明,650052
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| 摘要: |
| 采用测定粗品肝素纳260、280 nm光吸收和蛋白质的三氯乙酸浊度法,有效地解决了质量和效价的判断问题.结果显示,光吸收和蛋白质浊度与样品的效价成反比关系,可根据所归纳的光吸收和蛋白质浊度的6个程度,判断30～140 USP u/mg的样品,鉴定样品质量的优劣程度. |
| 关键词: 肝素 效价 光吸收 蛋白质浊度 |
| DOI： |
| 分类号:Q503 |
| 基金项目: |
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| Estimations of Quality and valence of Crude Sodium Heparin |
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| Abstract: |
| It is difficult to determine the quality and to estimate the valence range of crude sodium heparin by observing its color, appearance and hardness. However, through measuring the absorption of its solution at 260 and 280nm, and determining its turbidity and precipitate of protein ofter adding trichloroacetic acid, the above problems could be solved. It was found that the valence of crude sodium heparin was inversely proportional to the absorption of its solution at 260 and 280nm. The similar relationship between the valence and the turbidity was found. Six grades of crade sodium heparin could be estimated according to the absorption at 280 and the turbidity of its solution. |
| Key words: Heparin Potency Light Absorption Protein turbidity and precipitate |
