| 摘要: |
| 使用DNS-cl(Dimethylamino-naphthylane-5-sulfony chloride)荧光试剂,标记氨基酸成DNS-AAs(DNS_Amino Acids),然后采用聚酰胺薄膜层析,制成具有20余种DNS-AAs的荧光薄膜层析图谱,选择其中所需要检测的氨基酸,分别经洗脱后在日本岛津RF-510荧光分光光度计上,进行定量检测,分析了36例慢性肾炎患者,18例尿毒症患者血浆中12种游离氨基酸和35例慢性肾炎患者尿液中13种游离氨基酸,分别与29例正常人血浆和30例正常人尿液中游离氨基酸进行了比较,以此探讨肾脏疾病体液氨基酸模式的同时提供氨基酸治疗肾脏疾病的科学依据. |
| 关键词: 游离氨基酸 模式 肾脏疾病 |
| DOI： |
| 分类号:R692 |
| 基金项目: |
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| Pattern of free amino acids in body fluid from Patients with Kidney diseases |
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| Abstract: |
| Building pattern of free amino acids the body fluid from patients with kidney diseases had important significance. The results showed that (1)level of 10-12 free amino acids in plasma was siginificantly increased and molecular rato of Val/Gly、Try/Phe decreased in patients with chronic nephritis and uremia; (2)level of 10 free amino acids in urine was siginificantly increased in patients with chronic nephritis. It is explained that the pattern of free amino acid in body fluid is an important basis for diagnosis and for designing amino acid therapentics for curring kideny disease. |
| Key words: free amino acid pattern kideny disease |
