| 摘要: |
| 一点缀螟Paralipsa gularis(Zeller)是近年来在云南玉米产区发现的一种严重危害玉米果穗的害虫,为了明确其肠道可培养细菌对食物营养及次生代谢物质的代谢功能,本研究采用透明圈生物测定法结合高效液相色谱技术,筛选研究了一点缀螟幼虫肠道可培养淀粉、纤维素、蛋白质、阿魏酸、香草醛和对羟基苯甲酸的降解菌。结果表明,从一点缀螟肠道可培养细菌中,共筛选到18株分别具有淀粉、纤维素、蛋白质、阿魏酸、香草醛和对羟基苯甲酸降解力的细菌,其中对淀粉降解能力最强的为枯草芽孢杆菌(DH3)透明圈(Hp)和菌落直径(Dp)的比值为1.70；纤维素的降解能力最强的是苏云金芽孢杆菌(BS1),Hp/Dp为4.47；蛋白质降解能力最强的为粘质沙雷氏菌(BS5),Hp/Dp为3.46；阿魏酸的降解能力最强的是枯草芽孢杆菌(DH3),降解率达99.92％；香草醛的降解能力最强的是假格里尼翁布鲁氏菌(DH5),降解率达99.82％；腐生葡萄球菌(BS2和BS7)、平流层芽孢杆菌(BS4)、粘质沙雷氏菌(BS5)、蜡样芽孢杆菌(BS8)和假格里尼翁布鲁氏菌(DH5)降解对羟基苯甲酸的能力都很强,降解率均达100％。综上所述,一点缀螟肠道富含能降解玉米营养物质及玉米次生代谢物质的细菌,这些肠道细菌在一点缀螟的营养物质和次生代谢物质代谢中发挥了重要作用。该结果将为一点缀螟肠道细菌功能的深入研究及综合防治提供理论依据,同时为功能菌株的开发利用提供菌株资源。 |
| 关键词: 玉米 一点缀螟 肠道细菌 营养物质 次生代谢物质 降解功能 |
| DOI： |
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| 基金项目:云南省科技计划基础研究专项(202401AS070089),云南省教育厅科学研究(2023Y1045)。 |
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| Screening studies on culturable bacteria for degradation of food nutrients and secondary metabolites in the intestinal tract of Paralipsa gularis |
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姚云昊, Fu Qiao-si1, Zhao Guo-an2, Shen Yun-feng2, Wang Wen-qian1, Peng Yue-jin1, Du Guang-zu1
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1.College of Plant Protection,Yunnan Agricultural University/State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan;2.Plant Protection and Inspection Station of Baoshan city in Yunnan province
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| Abstract: |
| Paralipsa gularis (Zeller) is a serious pest of corn cob found in Yunnan maize production areas in recent years, in order to clarify the metabolic function of its intestinal culturable bacteria on food nutrients and secondary metabolites, in the present study, we used the thylakoid bioassay method combined with high-performance liquid chromatography (HPLC), to screen and study the degrading bacteria of the larval intestine of P.gularis that can be cultured for starch, cellulose, protein, ferulic acid, vanillin, and p-hydroxybenzoic acid. The results showed that a total of 18 bacterial strains were screened from P. gularis intestinal culturable bacteria with starch, cellulose, protein, ferulic acid, vanillin, and p-hydroxybenzoic acid degrading power, respectively Among them, the one with the strongest starch degrading ability was Bacillus subtilis (DH3) The ratio of hyaline circle (Hp) to colony diameter (Dp) was 1.70; The strongest degradation of cellulose was by Bacillus thuringiensis (BS1) with Hp/Dp of 4.47; The strongest protein degradation was in Serratia marcescens (BS5) with Hp/Dp of 3.46; The strongest degradation of ferulic acid was achieved by Bacillus subtilis (DH3) with a degradation rate of 99.92%; The strongest degradation of vanillin was by Brucella pseudogrignonii (DH5) with 99.82% degradation rate; Staphylococcus saprophyticus (BS2 and BS7), Bacillus stratosphericus (BS4), Serratia marcescens (BS5), Bacillus cereus (BS8), and Brucella pseudogrignonensis (DH5) degraded p-hydroxybenzoic acid with 100%. In summary, the intestinal tract of P.gularis is enriched with bacteria that can degrade maize nutrients and maize secondary metabolites, and these intestinal bacteria play an important role in the metabolism of nutrients and secondary metabolites in P.gularis. The results will provide a theoretical basis for the in-depth study of the functions of P.gularis intestinal bacteria and the comprehensive control of P.gularis, as well as provide strain resources for the development and utilization of functional bacterial strains. |
| Key words: Maize Paralipsa gularis(Zeller) Gut bacteria Nutrient Secondary metabolite Degradability |
