| 摘要: |
| 从源自红树根际土壤的真菌Mycosphaerella sp. HS-47发酵产物中分离鉴定具有抗菌活性的次生代谢产物。综合运用多种色谱方法,从乙酸乙酯萃取物中分离得到7个化合物,将其波谱数据与文献比对,分别鉴定为：ergost-7,9(11),22-trien-3β,5α,6α-triol (1)、stigmasta-7,22-diene-3β,5α,6α-triol (2)、6,9-epoxy-ergosta-7,22-dien-3-ol (3)、3β,5α,9α-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (4)、22E-7α-methoxy-5α,6α-epoxyergosta-8(14),22-dien-3β-ol (5)、5α,6α-epoxy-(22E,24R)-ergosta-8,22-dien-3β,7α-diol (6)和3β,5α-dihydroxy-10α-methyl-6β-acetoxy-ergosta-7,22-diene (7)。抗菌活性表明,1-7具有抗菌活性,其中5和6活性最为显著,对耐甲氧西林金黄色葡萄球菌S. aureus ATCC43300的最小抑制浓度均为1.56 μg.mL-1。本文首次报道从Mycosphaerella属中分离鉴定出甾体类化合物,且具有不同程度的抗菌活性。 |
| 关键词: Mycosphaerella sp. 麦角甾 分离鉴定 抗菌活性 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金 (81903772) |
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| Isolation and Identification of Ergosterols with Antibacterial Activityfrom the Fungus Mycosphaerella sp. HS-47 |
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HUA Silu, LIU Ping, MIAO Li
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College of Environmental Science and Engineering,Yangzhou University
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| Abstract: |
| The secondary metabolites with antibacterial activity were isolated and identified from the fermentation product of Mycosphaerella sp. HS-47 isolated from mangrove rhizosphere soil. Seven compounds were obtained from the EtOAc extract by means of various chromatographic techniques. The structures of the isolates were identified as ergost-7,9(11),22-trien-3β,5α,6α-triol (1), stigmasta-7,22-diene-3β,5α,6α-triol (2), 6,9-epoxy-ergosta-7,22-dien-3-ol (3), 3β,5α,9α-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (4), 22E-7α-methoxy-5α, 6α-epoxyergosta-8(14),22-dien-3β-ol (5), 5α,6α-epoxy-(22E,24R)-ergosta-8,22-dien-3β,7α-diol (6), and 3β,5α-dihydroxy-10α-methyl-6β-acetoxy-ergosta-7,22-diene (7), respectively, by comparison with spectroscopic data in the literature. 1-7 showed antibacterial activity, 5 and 6 were the most potent metabolites against S. aureus ATCC43300 with the minimum inhibitory concentration values at 1.56 μg.mL-1, respectively. The ergosterols isolated from Mycosphaerella were reported for the first time, and all of the isolates showed different level of antibacterial activity. |
| Key words: Mycosphaerella sp. ergosterols isolation and identification antibacterial activity |
