| 摘要: |
| 嗜麦芽寡养单胞菌（Stenotrophomonas maltophilia）是一种条件致病菌，其拥有多重耐药性。细菌素是由细菌合成的具有杀菌功能的蛋白质或蛋白聚合物，其中类噬菌体尾部细菌素的形态与肌尾噬菌体或长尾噬菌体的尾部相似，分为R型和F型。嗜麦芽菌素F1是一种新的F型类噬菌体尾部细菌素，由嗜麦芽寡养单胞菌c25合成。电镜观察显示其形态类似长尾噬菌体的尾部，双层平板法检测其杀菌活性最高达到64 000 AU/mL，能够裂解64株测试菌株中的32株。通过序列分析和基因敲除实验确定其基因簇由18个基因组成，这些基因的排列与Lambda噬菌体尾部结构基因的排列一致。这是首次分离到嗜麦芽寡养单胞菌合成的F型类噬菌体尾部细菌素，该细菌素有较强的杀菌能力和较广的杀菌谱，有望代替抗生素用于治疗嗜麦芽寡养单胞菌的感染。 |
| 关键词: 嗜麦芽寡养单胞菌 类噬菌体尾部细菌素 杀菌活性 基因组分析 |
| DOI：10.14188/j.ajsh.20240312002 |
| 分类号:Q933 |
| 基金项目:国家科技基础资源调查专项（2021FY100900）；国家大学生创新创业训练计划项目（202110486031） |
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| Characterization of a novel F-type maltocin and identification of its gene cluster |
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ZHOU Wei, LIU Zehui, LI Xinyu, LIU Liangyi, SHI Bozhang, WU Han, HUANG Yuping
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College of Life Sciences，Wuhan University，Wuhan 430072， Hubei， China
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| Abstract: |
| Stenotrophomonas maltophilia is an opportunistic pathogen， with multi-drug resistance. Bacteriocin is a kind of bactericidal protein or protein complex synthesized by bacteria， and the morphology of phage tail-like bacteriocin is similar to the phage tails of Myoviridae or Siphoviridae and can be divided into R-type and F-type. Maltocin F1 isolated in this study was detected as a new F-type phage tail-like bacteriocin synthesized by S. maltophilia c25. Transmission electron microscopy showed that its morphology was similar to the tail of Siphoviridae.The bactericidal activity of maltocin F1 was up to 64 000 AU/mL. Thirty-two strains of the 64 tested strains were sensitive to maltocin F1. Sequence analysis and gene knockout experiment predicted that the gene cluster of maltocin F1 was composed of 18 genes， the arrangement of which was consistent with that of the tail structural genes of phage lambda . This is the first time to isolate the F-type phage tail-like bacteriocin synthesized by S. maltophilia. Maltocin F1 has strong bactericidal ability and broad bactericidal spectrum， suggesting that it is a potent substitute of antibiotics in the treatment of S. maltophilia infections. |
| Key words: Stenotrophomonas maltophilia phage tail-like bacteriocin bactericidal activity genomic analysis |
