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| 热带药材虎乳灵芝质量标准建立 |
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张梓杰1,2,3，冯剑1,2,3，何明军1,2,3，徐林4，刘洋洋1,2,3
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1.中国医学科学院 药用植物研究所 海南分所， 海南 海口 570311;2.海南省南药资源保护与开发利用重点实验室， 海南 海口 570311;3.中药质量国际联合研究中心， 海南 海口 570311;4.中国热带农业科学院环境与植物保护研究所， 海南 海口 571101
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| 摘要: |
| 制定热带药材虎乳灵芝的质量标准。该研究对虎乳灵芝药材的性状、显微结构和薄层色谱特征进行了定性鉴别，参照《中国药典》2020年版测定其水分、总灰分、浸出物含量，并采用紫外分光光度法对总多糖等含量指标进行测定。规定虎乳灵芝药材水分含量不得超过13.0%、总灰分不得超过2.0%、水溶性浸出物含量不得少于3.0%；以无水葡萄糖作对照，虎乳灵芝药材总多糖含量不得少于0.45%。通过研究，建立了虎乳灵芝专属性薄层，创新了显微鉴别方法，并制定了虎乳灵芝药材标准。该研究方法重现性好，简便高效，可用于热带药材虎乳灵芝的质量控制，为进一步控制虎乳灵芝药材质量、研究与开发虎乳灵芝提供了参考。 |
| 关键词: 虎乳灵芝 质量标准 总多糖 |
| DOI：10.14188/j.ajsh.20240402001 |
| 分类号:R29 |
| 基金项目:海南省重大科技计划项目（ZDZX2021034）；万宁市科研项目（2022wnkj10） |
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| Establishment of quality standards for the tropical herbs Lignosus rhinocerus |
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ZHANG Zijie1,2,3, FENG Jian1,2,3, HE Mingjun1,2,3, XU Lin4, LIU Yangyang1,2,3
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1.Hainan Branch Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences， Haikou 570311， Hainan， China;2.Hainan Provincial Key Laboratory of Resources Conservation and;Development of Southern Medicine， Haikou 570311， Hainan， China;3.International Joint Research Center for Quality of Traditional Chinese Medicine， Haikou 570311， Hainan，China;4.Environment and Plant Protection Institute， Chinese Academy of Tropical Agriculture Sciences， Haikou 571101， Hainan， China
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| Abstract: |
| This study aims to establish the quality standard of Lignosus rhinocerus， a tropical medicinal herb. In this study， the qualitative identification of traits， microstructure and thin-layer chromatographic characteristics of Lignosus rhinocerus was carried out. The contents of water， total ash and extract contents of Lignosus rhinocerus were determined by according to the methods of Chinese Pharmacopoeia in 2020. UV spectrophotometric method was applied for the determination of total sugars content. The quality standards of Lignosus rhinocerus are that the moisture content shall not exceed 13.0%， the total ash shall not exceed 2.0%， and the content of water-soluble leachate shall not be less than 3.0%； and with dextrose anhydrous as the control， the total sugars content of Lignosus rhinocerus should not be less than 0.45%. The results of the study established a proprietary thin-layer identification method for Lignosus rhinocerus and derived the optimal extraction process for the total flavonoids of Lignosus rhinocerus The method is reproducible， simple and efficient， and can be used for the quality control of Lignosus rhinocerus. It provides a reference for the further control of the quality of the herbs of Lignosus rhinocerus， and for the research and development of Lignosus rhinocerus. |
| Key words: Lignosus rhinocerus quality standard total sugars |
