| 摘要: |
| 本研究利用筛选出的14对SSR引物,对4省5个地区的153份盐肤木种质资源进行遗传多样性分析,并进行遗传关系及聚类分析。结果显示14对SSR引物在153份样本中扩增到105个等位基因,平均每对引物扩增到的等位基因数为7.5个。盐肤木Nei’s多样性指数(H)0.206~0.413,平均值为0.316；Shannon多样性信息指数(I)0.356~0.600,平均值为0.484。5个居群间的有效等位基因数(Ne)1.335~1.556,Nei’s多样性指数(H)0.197~0.316,Shannon多样性信息指数(I)0.302~0.467,居群的遗传分化系数(Gst)变化范围0.122~0.489,平均值为0.257,基因流的变化范围(Nm)0.522~3.594,平均值为1.445(Nm＞1)。说明盐肤木在物种水平上遗传多样性丰富,居群间遗传多样性存在一定差异,总体变异的25.7%发生在居群间,74.3%的遗传变异发生在居群内,盐肤木的遗传分化的发生主要存在于居群内部,居群间存在着较频繁的基因交流。遗传多样性系数及聚类分析结果显示,5个居群被聚为两类,盐肤木优先以居群聚类,盐肤木的遗传关系与地理位置之间有一定的相关性。该研究结果可以对盐肤木后期优良种质的选育提供参考依据。 |
| 关键词: 盐肤木 SSR 遗传多样性 聚类分析 |
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| 基金项目:湖北省中央引导地方发科技发展专项(2022BGE265)、国家自然科学基金面上项目(NO32370393) |
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| Genetic Diversity Analysis of Rhus chinensis based on SSR Marker |
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Wu Wenwen,Yang Bingbing,Zhang Xia,Liu Wen,He Zhengquan
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Biotechnology Research Center of Three Gorges University,Key Laboratory of Plant Genetics and Germplasm Innovation in the Three Gorges Region,Yichang
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| Abstract: |
| To study the genetic diversity of Rhus chinensis Mill population and lay a theoretical foundation for the rational management, protection and utilization of Rhus chinensis germplasm resources. Methods: Using 14 pairs of SSR primers, the genetic diversity of 153 Rhus germplasm resources from 5 regions in 4 provinces was studied, the genetic diversity parameters were calculated, and the genetic relationship and cluster analysis were carried out. Results: (1) 14 pairs of SSR primers were amplified to 105 alleles in 153 samples, and the average number of alleles per pair was 7.5. (2) Nei"s diversity index (H) ranged from 0.206 to 0.413, with an average value of 0.316; The Shannon Diversity Information Index (I) ranged from 0.356 to 0.600, with an average value of 0.484. (3) The effective alleles (Ne), Nei"s diversity index (H) and Shannon diversity information index (I) were 1.335-1.556, 0.197-0.316 and 0.302-0.467 respectively. (4) The genetic differentiation coefficient (Gst) of the population was 0.257, and the inter-population gene flow was 1.445 (Nm > 1). (5) The coefficient of genetic diversity and cluster analysis showed that the 5 populations were grouped into two groups, and Rhus chinensis was preferentially grouped. Conclusion: The genetic diversity of Rhus chinensis is high, the genetic variation mainly exists within the population, and there is frequent gene exchange among the populations. The genetic relationship of Rhus Chinensis is related to the geographical location. The results of this study can provide reference for the breeding of excellent germplasm of Rhus chinensis in later period. |
| Key words: Rhus chinensis Mill, SSR , Genetic diversity, Cluster analysis |
