| 摘要: |
| 假单胞菌因其具有广谱抑菌活性，在植物病害生物防治中具有突出的潜力。对一株腐臭假单胞菌（Pseudomonas taetrolens）P683的防病能力、病原菌广谱抗性以及植物促生指标进行检测，进一步通过基因组测序及功能注释、antiSMASH预测了次级代谢物合成相关基因。结果表明菌株P683能够有效防治烟草野火病；具有广谱病原细菌抗性，能够拮抗植物病原细菌番茄青枯病菌（Ralstonia solanacearum）、番茄细菌性溃疡病菌（Clavibacter michiganensis）、水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae）、水稻条斑病菌（Xanthomonas oryzae pv. oryzicola）、十字花科黑腐病菌（Xanthomonas campestris pv. carnpestris）、番茄细菌性叶斑病菌（Pseudomonas syringae pv. tomato）以及人和/或动物病原细菌沙门氏菌（Salmonella sp.）和金黄色葡萄球菌（Staphylococcus aureus）；平板共培养发现，菌株P683与枯草芽胞杆菌相比具有更强的竞争能力；植物促生指标检测发现菌株P683具有水解无机磷和有机磷、产嗜铁素、产蛋白酶、产纤维素酶、分泌吲哚乙酸（IAA）和产NH3的能力。基因组测序显示菌株P683基因组大小为4.96 Mbp，编码4 477个蛋白；antiSMASH预测得到7个次级代谢物生物合成基因簇，编码产物包含可能发挥抑菌活性的含内酯蛋白酶抑制剂和芳基多烯，为进一步解析菌株P683的抑菌机制以及开发菌株P683作为一种新型生防菌剂奠定了基础。 |
| 关键词: 腐臭假单胞菌 拮抗 促生 生物防治 |
| DOI：10.14188/j.ajsh.2024.01.003 |
| 分类号:S43 |
| 基金项目:国家重点研发计划（2022YFD1901300）；贵州省烟草公司遵义市公司重点研发项目（2021XM03）；北京市现代农业产业体系（BAIC04-2023-12） |
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| Multifunctional characterization and genomic analysis of Pseudomonas taetrolens P683 |
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WANG Jing1, GU Ting1,2, ZHENG Sasa1,3, MA Wenxin4, ZHAO Dongmei4, WEI Hailei1
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1.State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China， Key Laboratory of Microbial Resources Collection and Preservation， Ministry of Agriculture and Rural Affairs， Institute of Agricultural Resources and Regional Planning， Chinese Academy of Agricultural Sciences， Beijing 100081， China;2.College of Plant Protection， Shandong Agricultural University， Taian 271018， Shandong， China;3.College of Horticulture and Landscape Architecture， Tianjin Agricultural University， Tianjing 300392， China;4.Xinjiang Huier Agricultural Group Co.， LTD.， Changji 831100， Xinjiang， China
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| Abstract: |
| Pseudomonas has outstanding potential in biological control of plant diseases because of its broad-spectrum antibacterial activity. In this study， a strain of Pseudomonas taetrolens P683 was tested for its disease prevention ability， pathogen broad-spectrum resistance and plant growth promoting index. The genes related to potential secondary metabolites were further analyzed by genome sequencing， functional annotation and antiSMASH prediction. The results showed that strain P683 could control tobacco wildfire disease effectively. Strain P683 could antagonize broad-spectrum pathogenic bacteria including Ralstonia solanacearum， Clavibacter michiganensis， Xanthomonas oryzae pv. oryzae， Xanthomonas oryzae pv. oryzicola， Xanthomonas campestris pv. carnpestris， Pseudomonas syringae pv. tomato， Salmonella sp.， and Staphylococcus aureu. Plate coculturing showed that strain P683 had stronger competitive capacity than Bacillus subtilis. Plant growth promoting index detection showed that strain P683 could hydrolyze inorganic and organic phosphorus， produce siderophore， protease， cellulase， IAA， and NH3. Genome sequencing revealed that the genome size of strain P683 is 4.96 Mbp， encoding 4 477 proteins. Seven secondary metabolite biosynthetic gene clusters were predicted by antiSMASH， encoding products containing lactone protease inhibitors and arylpolyenes that may exert antibacterial activity. This study lays the foundation for further analysis of the bactericidal mechanism of strain P683 and development of strain P683 as a new biocontrol agent. |
| Key words: Pseudomonas taetrolens antagonism growth promoting biocontrol |
