| 摘要: |
| 摘要：海南岛众多独立入海的河流形成天然的生物地理屏障，造就了海南丰富的淡水鱼类资源。李氏吻虾虎鱼(Rhinogobius leavelli (Herre,1935)) 因利用价值低而未被人工大量养殖驯化，可作为评估鱼类遗传多样性的理想物种。本文基于COX1和D-loop分子标记，分析了海南岛南渡江、珠碧江、三亚河和陵水河等4条河流中李氏吻虾虎鱼的种群遗传结构。遗传多样性分析显示，4条水系中43尾李氏吻虾虎鱼COX1、D-loop及联合基因中分别检测出8、14、22个变异位点和6、7、8个单倍型，平均单倍型多样性和核苷酸多样性分别是0.617、0.647、0.649和0.0039、0.0094、0.0063，其中三亚河与陵水河种群的遗传多样性均较高。系统发育树结果均显示，李氏吻虾虎鱼4个水系的5个地理种群聚类为2个进化分支，其中三亚河与陵水河种群聚为一支，南渡江、珠碧江种群聚为一支。遗传距离分子方差分析显示，李氏吻虾虎鱼群体的遗传变异主要来自种群间。上述结果表明，海南岛李氏吻虾虎鱼表现出显著的遗传分化，遗传多样性水平较高，可能因不同水系的隔离效应和李氏吻虾虎鱼较弱的迁移能力导致其种群间的遗传分化，本文为海南岛河流生态系统中鱼类种质资源多样性的研究提供遗传学参考。 |
| 关键词: 海南岛 李氏吻虾虎鱼 遗传多样性 遗传结构 |
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| 基金项目:海南省重点研发计划 (ZDYF2023SHFZ132; ZDYF2021XDNY299); 海南省自然科学基金高层次人才项目(323RC554) |
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| Genetic Structure of Rhinogobius leavelli (Herre,1935) within Four Rivers of Hainan Island |
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Zhangqingfeng, Shenzhixin, Lifangyuan, Ligaojun, Guyuan, Dongyang, Caixingwei
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Academic Of Ocean And Fisheries Of Hainan Province
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| Abstract: |
| Abstract: Numerous independent rivers form a natural biogeographical barrier, creating a rich freshwater fish resources in Hainan island. R. leavelli has not been domesticated in large numbers because of its low utilization value, so it can be used as an ideal species to evaluate the genetic diversity of fish. Based on COX1 and D-loop molecular markers, the genetic structure of R. leavelli within Nandu, Zhubi, Sanya and Linshui rivers was analyzed. Genetic diversity analysis showed that 8, 14, 22 variation sites and 6, 7, 8 haplotypes were detected respectively in COX1, D-loop and combined genes of the 43 R. leavelli within four rivers. The average haplotype diversity and nucleotide diversity were 0.617, 0.647, 0.649 and 0.0039, 0.0094, 0.0063, respectively. The genetic diversity of Sanyahe and Lingshuihe populations was higher than other pupulations. Phylogenetic tree indicathe that 5 populations within four rivers clusters with 2 evolutionary branches, among which the population of Sanya River and Lingshui River were clusters together and the Nandu River and Zhubi River were clusters together. Molecular variance showed that the genetic variation of R. leavelli within four rivers was mainly occured inter-population. These results above indicate that the genetic differentiation of R. leavelli in Hainan Island is significant and the level of genetic diversity is high, which may be caused by the isolation effect of different water systems and the weak migration ability of R. leavelli. This paper provides a genetic reference for the study of fish genetic diversity in the river ecosystem of Hainan Island. |
| Key words: Hainan Island Rhinogobius leavell genetic diversity Genetic structure |
