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设施黄瓜连作土壤对黄瓜枯萎病菌致病力的影响
李世贵,曾文,刘快,国振宇,赵晓迪,顾金刚
中国农业科学院农业资源与农业区划研究所,北方干旱半干旱耕地高效利用全国重点实验室/农业农村部农业微生物资源收集与保藏重点实验室,北京100081
摘要:
为系统研究设施蔬菜连作障碍的发生机制,探讨连作年限对蔬菜土传病害病原菌致病力的影响。本文以山东省德州市王杲铺镇看水庄村不同连作年限的设施黄瓜大棚中采集的土壤样品为研究对象,通过测定其土壤理化性质分析土壤次生盐渍化的程度,并用不同连作年限土壤进行盆栽实验,分析黄瓜植株的生长情况、发病情况及黄瓜枯萎病菌在黄瓜植株内和根际土壤中的定殖情况,研究不同连作年限土壤对黄瓜枯萎病菌致病力的影响。结果表明,所有土壤样品的pH均在8.0以上,呈碱性;土壤样品的全磷含量与pH变化趋势一致;连作8年土壤中黄瓜枯萎病菌在黄瓜植株茎部的定殖数量最多,黄瓜植株的病情指数和发病率最高,说明黄瓜枯萎病菌在黄瓜植株茎部定殖数量多少与黄瓜植株的病情指数和发病率高低的变化趋势一致。综上所述,本研究为设施蔬菜土壤土传病害的防控提供了理论依据。
关键词:  连作土壤  土传病害  黄瓜枯萎病菌  致病力  次生盐渍化
DOI:10.14188/j.ajsh.2023.06.010
分类号:S154.36
基金项目:国家重点研发计划项目(2017YFD0200604-5)
Effect of continuous cropping soil of greenhouse cucumber on the pathogenicity of Fusarium oxysporium f. sp. cucumberinum
LI Shigui, ZENG Wen, LIU Kuai, GUO Zhenyu, ZHAO Xiaodi, GU Jingang
State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract:
In order to systematically study the occurrence mechanism of vegetable continuous cropping obstacles in facilities and explore the effect of continuous cropping years on the pathogenicity of vegetable soil-borne diseases. In this study, the soil samples were collected from the different continuous cropping cucumber greenhouses in Kanshuizhuang Village, Wanggaopu Town, Dezhou City, Shandong Province. The degree of soil secondary salinization was analyzed by measuring the soil physical and chemical properties. The growth, disease incidence and the colonization of Fusarium oxysporium f. sp. cucumberinum were analyzed by pot experiments, and effects of the continuous cropping soil on its pathogenicity were studied. The results showed that the pH of all soil samples was above 8.0, indicating alkalinity. The total phosphorus content of soil samples was consistent with the trend of pH changes. In the soil of continuous cropping for 8 years, the number of the pathogens colonized the stem of cucumber plants was the largest, and the disease index and incidence rate of cucumber plants were the highest, indicating that the number of the pathogens colonized the stem of cucumber plants is consistent with the change trend of disease index and incidence rate of cucumber plants. This study provides a theoretical basis for the prevention and control of soil-borne diseases of facility vegetables in the future.
Key words:  continuous cropping soil  soil-borne disease  Fusarium oxysporium f. sp. cucumberinum  pathogenicity  secondary salinization