| 摘要: |
| 金线兰属植物形态相似，峨眉金线兰（Anoectochilus emeiensis）为金线兰（Anoectochilus roxburghii）的近缘种。调查发现，市场上常将峨眉金线兰当作金线兰使用，为区分两者，本试验采用生药学鉴定方法观察峨眉金线兰的原植物形态、组织构造及粉末显微特征；采用HPLC-ELSD法测定峨眉金线兰中主要内酯苷的含量。峨眉金线兰叶片为卵形，具有金红色网纹；花不倒置，唇瓣白色，前部裂片长圆状倒披针形，中部两侧各具1枚宽大长圆形的褶片且其边缘具疏锯齿。显微结构中，根茎横切面中皮层宽广，根木质部导管2~5个成束，茎木质部导管1~8个成束；叶横截面叶肉细胞中有大量红色物质，上表皮呈乳突状突起，下表皮气孔较多，气孔以不定式为主；粉末中可见草酸钙针晶和导管。3批峨眉金线兰中金线莲苷和斑叶兰苷（goodyeroside A）的含量分别为32.66~35.21 mg/g和134.41~137.72 mg/g，分别在100~500 μg/mL和200~1 000 μg/mL质量浓度范围内呈良好的线性关系，平均加样回收率分别为100.51%（RSD=1.56%）和97.50%（RSD=1.54%）。以上特征可为峨眉金线兰的生药鉴别提供参考依据。 |
| 关键词: 峨眉金线兰 生药鉴别 高效液相蒸发散射法 含量测定 |
| DOI：10.14188/j.ajsh.2023.06.008 |
| 分类号:R931.5 |
| 基金项目:福建省科技厅高校产学合作项目（2020Y4015） |
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| Pharmacognostic identification of Anoectochilus emeiensis |
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HAN Yu1, WANG Shuoshuo1, PENG Mengchao1, CHEN Huiling1, ZHENG Chengjian2, WU Yanbin1, WU Jinzhong1
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1.College of Pharmacy， Fujian University of Traditional Chinese Medicine， Fuzhou 350122， Fujian， China;2.College of Pharmacy，Naval Medical University， Shanghai 200433， China
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| Abstract: |
| Anoectochilus emeiensis is a closely related species of Anoectochilus roxburghii. According to the investigation， A.emeiensis is used as A.roxburghii in the market. In order to distinguish the two， the original plant morphology， tissue structure and powder microscopic characteristics of Anoectochilus emeiensis were observed by pharmacognosy identification method. HPLC-ELSD method was used to determine the contents of main lactone glycosides in Anoectochilus emeiensis. The leaves are ovate， with golden red mesh pattens； the flowers are not inverted， with white lips and long round lanceolate anterior lobes. On both sides of the middle， there is a wide， elongated pleat with sparse serrations at the edges. In the microstructure， the cortex in the transverse section of the rhizome is broad， with bundles of 2-5 vessels in the root xylem and bundles of 1-8 vessels in the stem xylem. There is a large amount of red substance in the mesophyll cells of the leaf cross section， the upper epidermis has mastoid protrusions， and the lower epidermis has many stomata， mainly infinitive stomata. Calcium oxalate needle crystal and catheter can be seen in the plant powder. The contents of kinsenoside and goodyeroside A in 3 batches of Anoectochilus emeiensis were 32.66-35.21 mg/g and 134.41-137.72 mg/g， respectively， and showed a good linear relation between 100-500 μg/mL and 200-1 000 μg/mL， respectively. The average recovery rates of kinsenoside and goodyeroside A were 100.51% （RSD=1.56%） and 97.50% （RSD=1.54%），respectively.These characteristics can provide reference for the pharmacognostic identification of Anoectochilus emeiensis. |
| Key words: Anoectochilus emeiensis pharmacognostic identification HPLC-ELSD method content determination |
