| 摘要: |
| 为系统了解北京草莓种植区土壤微生物组群落动态与土壤质量的关系,本研究利用高通量测序,系统分析北京地区草莓根际微生物组,结果显示细菌群落结构分布具有明显的区域性,磷元素和钾元素是影响草莓根际菌群组成最主要的因子；扩增子测序分析表明,健康植株比发病植株根际土壤群落结构更为稳定；网络分析发现了潜在的与草莓土壤健康相关的细菌菌属,其中发病样品中链霉菌属和芽孢菌属占据重要位置。进一步对比草莓健康与发病根际微生物组成的差异,探究了病害发生与根际细菌群落变化之间的规律,并通过构建相关性网络发现了潜在的病害防治菌属,为北京草莓土传病害预警和防控奠定了基础。 |
| 关键词: 扩增子测序 微生物组 草莓枯萎病 根际微生物 土壤理化性质 |
| DOI： |
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| 基金项目:特色作物产业技术体系北京市创新团队(BAIC04-2022)；北京市乡村振兴科技项目(BJXCZX2022) |
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| Deciphering the soil microbiome of strawberry planting areas in Beijing |
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MA Yinan#, ZENG Wen#, MA Rong, ZHANG Xiaoxia, LI Shigui, WEI Hailei, GU Yilin
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Institute of Agricultural Resources and Regional Planning,Key Laboratory of Microbial Resources Collection and Preservation,Ministry of Agriculture and Rural Affairs
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| Abstract: |
| To systematically learn the relationship between soil microbiome community dynamics and soil quality in strawberry plantation area of Beijing, this study used high-throughput sequencing, and high-comparatively analyzed the rhizosphere microbiome of strawberry in Beijing. The results showed that the microbial composition was largely affected by geographic areas, and phosphorus and potassium are two major factors in shaping microbiome. Amplicon sequencing analysis shows that the bacterial composition in the healthy plants’ rhizosphere is more stable than that of diseased plants’ rhizosphere. Co-occurrence network analysis identified bacterial genera potentially associated with strawberry health, with Streptomyces sp. and Bacillus sp. occupying significant positions in the diseased samples. This study further compared the differences in microbial composition between healthy and diseased strawberry rhizosphere, explored the patterns between disease occurrence and changes in rhizosphere bacterial communities, and identified potential disease control genera by constructing co-occurrence networks, which laid the foundation for early warning and prevention and control of soil-borne diseases of strawberry in Beijing. |
| Key words: Amplicon sequencing Microbiome Strawberry fusarium wilt Rhizosphere microorganisms Soil physiochemical properties |
