| 摘要: |
| 靛蓝色素是人类所知最古老的色素之一，广泛用于食品、医药和印染工业。靛蓝最初由植物中提取获得，作为一种色泽艳丽、无毒无害、能够生物降解的环保型染料，深受青睐。生物合成靛蓝因其绿色、高效、节约土地、安全稳定等优点，作为取代目前植物提取及化学合成的主流途径，受到广泛关注。在过去的几十年中，许多天然酶和工程酶已筛选用于合成靛蓝。本文概述了能够催化靛蓝生物合成的酶及相关应用，并探讨了当前存在的问题和未来研究方向，为推进基于生物合成的靛蓝工业化生产奠定基础。 |
| 关键词: 靛蓝 色素 酶 生物合成 催化机制 |
| DOI：10.14188/j.ajsh.2023.04.004 |
| 分类号:Q814 |
| 基金项目:国家自然科学基金项目（32170402，81874335）；重点研发计划“中药现代化专项”（2022YFC3501700）；上海市优秀学术带头人计划（23XD1423500） |
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| Research advances in enzymes involved in indigo biosynthesis |
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LI Rongrong1, MA Wenhao2, XIAO Ying1
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1.The Shanghai University of Traditional Chinese Medicine Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine， Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine， Shanghai 201203， China;2.The Eighth People’s Hospital of Shanghai， Shanghai 200235， China
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| Abstract: |
| Indigo is one of the oldest pigments known to mankind and is widely used in the food， medicine and printing-dyeing industries. Indigo was originally extracted from plants and is highly favored as an environmentally friendly dye with bright color， non-toxic， and biodegradable properties. Biosynthesis of indigo has attracted widespread attention as a mainstream way to replace the current plant extraction and chemical synthesis due to its advantages of green， efficient， landsaving， safety and stability. Over the past few decades， a number of natural and engineered enzymes have been identified for use in the synthesis of indigo. This review summarizes the enzymes that can catalyze indigo biosynthesis and their related applications， and discusses the current problems and future research directions， laying the foundation for promoting the industrial production of indigo based on biosynthesis. |
| Key words: indigo pigment enzyme biosynthesis catalytic mechanism |
