| 摘要: |
| 探讨柯萨奇病毒A组10型（coxsackievirus A10， CV-A10）感染人横纹肌肉瘤（rhabdomyosarcoma，RD）细胞的基因表达谱变化，为深入理解CV-A10致病机制提供重要基础数据。本研究将CV-A10以感染复数（MOI）为0.1的剂量感染RD细胞12 h后提取总RNA，通过RNA-Seq技术获取RD细胞中的全部转录本信息，基于生物信息学的方法对差异表达基因进行GO（gene ontology， GO）和KEGG（Kyoto encyclopedia of genes and genomes， KEGG）分析。结果显示，共筛选到2 610个差异表达基因，其中表达上调的基因有1 582个，表达下调的基因有1 028个。随机选取8个差异表达基因，经RT-qPCR验证后发现，其变化趋势与转录组数据一致。GO和KEGG富集分析结果显示，差异表达基因主要聚焦于细胞内信号转导、mRNA加工、抗病毒免疫反应等过程。结果表明，CV-A10感染可能会引起RD细胞发生自噬。 |
| 关键词: 柯萨奇病毒A组10型 人横纹肌肉瘤细胞 转录组学 |
| DOI：10.14188/j.ajsh.2023.02.010 |
| 分类号:R373.2 |
| 基金项目:国家自然科学基金（81660280） |
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| Transcriptomics analysis of RD cells infected with coxsackievirus A10 |
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JIANG Juntao1,2, REN Yingying3, LIU Hongbo1,2
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1.Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders， The Second Affiliated Hospital of Guilin Medical University， Guilin 541199， Guangxi， China;2.College of Clinical Laboratory Science of Guilin Medical University， Guilin 541199， Guangxi， China;3.State Key Laboratory of Virology， College of life sciences at Wuhan University， Wuhan 430072， Hubei， China
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| Abstract: |
| To explore the changes of gene expression profile in human rhabdomyosarcoma （RD） cells infected with coxsackievirus A10 （CV-A10）， and to provide important basic data for further understanding the pathogenesis of CV-A10， RD cells were infected with CV-A10 at a multiplicity of infection （MOI） of 0.1 for 12 h， and total RNA was extracted. All transcriptional information of infected RD cells was obtained though RNA-Seq technology. Based on bioinformatics methods， differentially expressed genes were analyzed using GO （gene ontology） and KEGG （Kyoto encyclopedia of genes and genomes）. The results showed that a total of 2 610 differentially expressed genes were screened， of which 1 582 were up-regulated and 1 028 were down-regulated. 8 differentially expressed genes were randomly selected and verified by RT-qPCR， and the variation trend was consistent with the transcriptome data. The enrichment analysis results of GO and KEGG showed that the differentially expressed genes mainly focused on intracellular signal transduction， mRNA processing， antiviral immune response， and other processes. This study suggests that CV-A10 infection may cause autophagy in RD cells. |
| Key words: coxsackievirus A10 human rhabdomyosarcoma cell transcriptomics |
