| 摘要: |
| 灵菌红素是由微生物产生的一种红色次级代谢产物，因其具有抗菌、抗癌和抗疟疾等功效，受到了生物医药领域的广泛关注。微生物发酵是当前产灵菌红素的主要方法，分离筛选高产灵菌红素的微生物、优化发酵条件是提高灵菌红素产率的重要途径。本研究从深圳湾筛选出一株含有红色色素的菌株，并基于16S rRNA基因序列对该菌株进行了系统发育分析和物种鉴定。紫外可见光全波长扫描和HPLC-MS图谱分析证明，该菌株所产红色色素为灵菌红素。进一步通过单因素试验和正交优化法优化了该菌产灵菌红素的发酵条件和发酵培养基组分。系统发育分析表明，该菌株为一株海洋粘质沙雷氏菌（Serratia marcescens），并将其命名为S. marcescens SOCE 001。产灵菌红素的最佳发酵条件为：温度28 ℃、振荡培养转速220 r/min、培养基pH 7。发酵培养基最佳组合为：果糖添加量2 g/L、蛋白胨添加量10 g/L，MgSO4添加量2 g/L。优化发酵条件后，经24 h培养， 灵菌红素产量可达2.468 g/L。 |
| 关键词: 粘质沙雷氏菌 次级代谢产物 灵菌红素 微生物发酵优化 |
| DOI：10.14188/j.ajsh.2022.06.003 |
| 分类号:TQ920. |
| 基金项目:国家自然科学基金（91851210）；广东省基础与应用基础研究基金（2021B1515120080）；近海海洋环境科学国家重点实验室（厦门大学）访问学者与开放课题基金（MELRS2210）；前沿海洋技术三维一体环境监测实验教学示范中心（SJZLGC202111） |
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| Fermentation optimization of a new prodigiosin-producing marine Serratia marcescens strain |
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GUO Jing1, XU Shuaishuai1, WANG Yidong1, ZHANG Chuanlun1,2,3, HOU Shengwei1,2,3
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1.Department of Ocean Science and Engineering， Southern University of Science and Technology， Shenzhen 518055， Guangdong， China;2.Shenzhen Key Laboratory of Marine Archaea Geo-Omics， Southern University of Science and Technology， Shenzhen 518005， Guangdong， China;3.Southern Marine Science and Engineering Guangdong Laboratory （Guangzhou）， Guangzhou 511458， Guangdong， China
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| Abstract: |
| Prodigiosin is a red-colored secondary metabolite naturally produced by diverse microbes， which has drawn much attention from the biomedical field due to its antimicrobial， anti-cancer and antimalarial activities. Prodigiosin is mainly produced by microbial fermentation， and selecting high-yield microbial strains or optimizing fermenting conditions are the two major approaches to improve prodigiosin production. In this study， we isolated a red pigment-producing strain from Shenzhen Bay， which was identified as Serratia marcescens based on 16S rRNA gene phylogenetic analysis. Ultraviolet-visible （UV-Vis） spectrophotometry and high performance liquid chromatography-mass spectrometry （HPLC-MS） confirmed that the red pigment was prodigiosin. To improve the yield of prodigiosin， the fermentation conditions and medium composition were further optimized using single factor and orthogonal experiments. Phylogenetic analysis revealed that this strain was a marine Serratia marcescens， and was named S. marcescens SOCE 001. The optimal fermentation conditions for prodigiosin production were at 28 ℃， with 220 rpm of shaking cultivation， and in a culture medium with pH 7. The optimal culture medium should be composed of 2 g/L of fructose， 10 g/L of peptone， and 2 g/L of MgSO4. Under the optimized medium and culture conditions the yield of prodigiosin can reach 2.468 g/L after 24 h of culture. |
| Key words: Serratia marcescens secondary metabolite prodigiosin microbial fermentation optimization |
