| 摘要: |
| 为了进一步探究传统藏药植物多刺绿绒蒿（Meconopsis horridula）中代谢物成分以及不同器官差异情况，采用UPLC-MS技术对多刺绿绒蒿的叶、根和花三个不同器官代谢物进行分析与鉴定。并利用主成分分析（PCA）、聚类热图分析、正交偏最小二乘-判别分析（OPLS-DA）和KEGG通路富集分析等方法进行不同器官差异代谢产物筛选与通路分析。结果显示，在ESI+和ESI-模式下，共检测注释到947种代谢物，不同器官间差异代谢物进行分析，叶和根差异代谢物有301个，叶和花中差异代谢物有170个，根和花中差异代谢物有244个。通过聚类热图可以看出，大多数代谢物在根中含量较低；KEGG通路富集分析显示，差异代谢物大多富集在氨基酸代谢、花青素生物合成、黄酮类生物合成和生物碱合成等代谢途径。各器官优势黄酮类、萜类和生物碱类代谢物的分析为进一步探究多刺绿绒蒿的不同器官药用特征成分和开发利用提供一定的帮助。 |
| 关键词: 多刺绿绒蒿 非靶代谢组学 藏药植物 黄酮类 萜类 差异代谢物 |
| DOI：10.14188/j.ajsh.2022.05.008 |
| 分类号:R932 |
| 基金项目:中央引导地方项目，藏药多刺绿绒蒿黄酮类代谢产物及其生物合成调控机制的研究（XZ202102YD0031C） |
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| Analysis of metabolites in different organs of Meconopsis horridula based on untargeted metabolomics |
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DING Yali
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College of Medicine， Tibet University， Lhasa 850000， Tibet， China
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| Abstract: |
| In order to further interrogate the difference between the different organ tissues in the traditional Tibetan medicinal plant Meconopsis horridula， its three parts （flower， leaf， and root） were identified and quantified by UPLC-MS/MS. Principal component analysis （PCA）， cluster heat map analysis， orthogonal partial least-squares discriminant analysis （OPLS-DA）， and KEGG pathway enrichment analysis were used for differential metabolite screening and pathway analysis. There were 947 metabolites detected by non-targeted metabolomics in two analytical modes ［ESI+ and ESI-］， of which 301 differed statistically between leaves and roots， 244 differed statistically between roots and flowers， and 170 differed statistically between leaves and flowers. From the cluster heatmap results， most metabolites are low in the roots. Furthermore， enrichment analyses of KEGG pathways for differentially expressed genes revealed that most differential metabolites are enriched in amino acid metabolism， anthocyanin biosynthesis， flavonoid biosynthesis， and alkaloid biosynthesis. The differential metabolites （flavonoids， terpenoids and alkaloids） in different organs were analyzed， and these analyses provide some help for further exploring the medicinal characteristic components of Meconopsis horridula and their development and utilization. |
| Key words: Meconopsis horridula untargeted metabolomics Tibetan medicinal plant flavonoid terpene differential metabolite |
